TY - JOUR
T1 - Hair-bundle proteomes of avian and mammalian inner-ear utricles
AU - Wilmarth, Phillip A.
AU - Krey, Jocelyn F.
AU - Shin, Jung Bum
AU - Choi, Dongseok
AU - David, Larry L.
AU - Barr-Gillespie, Peter G.
N1 - Funding Information:
This work was supported by NIH grants R01 DC002368, R01 DC011034, and P30 DC005983 to P.G.B.-G.; F32 DC012455 to J.F.K.; and P30 EY010572 and EY007755 to P.A.W. and L.L.D. Mass spectrometry carried out for this project was conducted at the OHSU Proteomics Shared Resource.
PY - 2015/12/8
Y1 - 2015/12/8
N2 - Examination of multiple proteomics datasets within or between species increases the reliability of protein identification. We report here proteomes of inner-ear hair bundles from three species (chick, mouse, and rat), which were collected on LTQ or LTQ Velos ion-trap mass spectrometers; the constituent proteins were quantified using MS2 intensities, which are the summed intensities of all peptide fragmentation spectra matched to a protein. The data are available via ProteomeXchange with identifiers PXD002410 (chick LTQ), PXD002414 (chick Velos), PXD002415 (mouse Velos), and PXD002416 (rat LTQ). The two chick bundle datasets compared favourably to a third, already-described chick bundle dataset, which was quantified using MS1 peak intensities, the summed intensities of peptides identified by high-resolution mass spectrometry (PXD000104; updated analysis in PXD002445). The mouse bundle dataset described here was comparable to a different mouse bundle dataset quantified using MS1 intensities (PXD002167). These six datasets will be useful for identifying the core proteome of vestibular hair bundles.
AB - Examination of multiple proteomics datasets within or between species increases the reliability of protein identification. We report here proteomes of inner-ear hair bundles from three species (chick, mouse, and rat), which were collected on LTQ or LTQ Velos ion-trap mass spectrometers; the constituent proteins were quantified using MS2 intensities, which are the summed intensities of all peptide fragmentation spectra matched to a protein. The data are available via ProteomeXchange with identifiers PXD002410 (chick LTQ), PXD002414 (chick Velos), PXD002415 (mouse Velos), and PXD002416 (rat LTQ). The two chick bundle datasets compared favourably to a third, already-described chick bundle dataset, which was quantified using MS1 peak intensities, the summed intensities of peptides identified by high-resolution mass spectrometry (PXD000104; updated analysis in PXD002445). The mouse bundle dataset described here was comparable to a different mouse bundle dataset quantified using MS1 intensities (PXD002167). These six datasets will be useful for identifying the core proteome of vestibular hair bundles.
UR - http://www.scopus.com/inward/record.url?scp=84960959991&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84960959991&partnerID=8YFLogxK
U2 - 10.1038/sdata.2015.74
DO - 10.1038/sdata.2015.74
M3 - Article
C2 - 26645194
AN - SCOPUS:84960959991
SN - 2052-4463
VL - 2
JO - Scientific Data
JF - Scientific Data
M1 - 150074
ER -