High efficiency gene delivery into laryngeal muscle with bidirectional electroporation

Koichiro Saito; Mohamed Lehar; Zhao Bo Li; Natasha Braga; Alan D. King; Robin A. Samlan; Paul W. Flint

doi:10.1016/j.otohns.2006.04.003

High efficiency gene delivery into laryngeal muscle with bidirectional electroporation

Koichiro Saito
, Mohamed Lehar
, Zhao Bo Li
, Natasha Braga
, Alan D. King
, Robin A. Samlan
, Paul W. Flint

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Objective: The impact of polarity change on the efficiency of in vivo electroporative (EP) gene transfection was assessed in rat laryngeal muscle. Study design and setting: High (HV) and low field voltage (LV) were combined with polarity changes to determine transfection in 5 different conditions: 1) without EP (EP[-]), 2) HV+LV (HL), 3) HV+LV followed by HV+LV with no change in polarity (HLHL unidirectional), 4) HV+LV followed by HV+LV with opposite polarity (HLHL bidirectional), 5) HV+LV followed by LV with opposite polarity (HLL bidirectional). Results: HLL bidirectional sequence showed the best result with less interindividual variability and extended expression period. With the exception of repeated high voltage sequences, EP parameters were not likely to induce cell injury or inflammation. Conclusion: HLL bidirectional electroporative gene delivery produces high transfection rates with limited tissue trauma. Significance: Bidirectional EP provides a safe and highly efficient method for therapeutic gene delivery into skeletal muscle.

Original language	English (US)
Pages (from-to)	209-214
Number of pages	6
Journal	Otolaryngology - Head and Neck Surgery
Volume	135
Issue number	2
DOIs	https://doi.org/10.1016/j.otohns.2006.04.003
State	Published - Aug 6 2006
Externally published	Yes

Funding

This work is funded by NIH/NIDCD grant DC04324-05.

Funders

Funder number

Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health

National Institute on Deafness and Other Communication Disorders R01DC012546 Xue Zhong Liu National Institute on Deafness and Other Communication Disorders R01DC012115 Xue Zhong Liu National Institute on Deafness and Other Communication Disorders R01DC014427 Ulrich Müller Peter G Barr-Gillespie National Institute on Deafnessand Other Communication Disorders R01DC005965 Ulrich Müller National Institute on Deafness and Other Communication Disorders

R01DC004324, DC04324-05

ASJC Scopus subject areas

Surgery
Otorhinolaryngology

Access to Document

10.1016/j.otohns.2006.04.003

Cite this

@article{9530abe20ced4bfbb5f62ff38fbda412,

title = "High efficiency gene delivery into laryngeal muscle with bidirectional electroporation",

abstract = "Objective: The impact of polarity change on the efficiency of in vivo electroporative (EP) gene transfection was assessed in rat laryngeal muscle. Study design and setting: High (HV) and low field voltage (LV) were combined with polarity changes to determine transfection in 5 different conditions: 1) without EP (EP[-]), 2) HV+LV (HL), 3) HV+LV followed by HV+LV with no change in polarity (HLHL unidirectional), 4) HV+LV followed by HV+LV with opposite polarity (HLHL bidirectional), 5) HV+LV followed by LV with opposite polarity (HLL bidirectional). Results: HLL bidirectional sequence showed the best result with less interindividual variability and extended expression period. With the exception of repeated high voltage sequences, EP parameters were not likely to induce cell injury or inflammation. Conclusion: HLL bidirectional electroporative gene delivery produces high transfection rates with limited tissue trauma. Significance: Bidirectional EP provides a safe and highly efficient method for therapeutic gene delivery into skeletal muscle.",

author = "Koichiro Saito and Mohamed Lehar and Li, \{Zhao Bo\} and Natasha Braga and King, \{Alan D.\} and Samlan, \{Robin A.\} and Flint, \{Paul W.\}",

note = "Funding Information: This work is funded by NIH/NIDCD grant DC04324-05. ",

year = "2006",

month = aug,

day = "6",

doi = "10.1016/j.otohns.2006.04.003",

language = "English (US)",

volume = "135",

pages = "209--214",

journal = "Otolaryngology - Head and Neck Surgery",

issn = "0194-5998",

publisher = "John Wiley and Sons Inc.",

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T1 - High efficiency gene delivery into laryngeal muscle with bidirectional electroporation

AU - Saito, Koichiro

AU - Lehar, Mohamed

AU - Li, Zhao Bo

AU - Braga, Natasha

AU - King, Alan D.

AU - Samlan, Robin A.

AU - Flint, Paul W.

N1 - Funding Information: This work is funded by NIH/NIDCD grant DC04324-05.

PY - 2006/8/6

Y1 - 2006/8/6

N2 - Objective: The impact of polarity change on the efficiency of in vivo electroporative (EP) gene transfection was assessed in rat laryngeal muscle. Study design and setting: High (HV) and low field voltage (LV) were combined with polarity changes to determine transfection in 5 different conditions: 1) without EP (EP[-]), 2) HV+LV (HL), 3) HV+LV followed by HV+LV with no change in polarity (HLHL unidirectional), 4) HV+LV followed by HV+LV with opposite polarity (HLHL bidirectional), 5) HV+LV followed by LV with opposite polarity (HLL bidirectional). Results: HLL bidirectional sequence showed the best result with less interindividual variability and extended expression period. With the exception of repeated high voltage sequences, EP parameters were not likely to induce cell injury or inflammation. Conclusion: HLL bidirectional electroporative gene delivery produces high transfection rates with limited tissue trauma. Significance: Bidirectional EP provides a safe and highly efficient method for therapeutic gene delivery into skeletal muscle.

AB - Objective: The impact of polarity change on the efficiency of in vivo electroporative (EP) gene transfection was assessed in rat laryngeal muscle. Study design and setting: High (HV) and low field voltage (LV) were combined with polarity changes to determine transfection in 5 different conditions: 1) without EP (EP[-]), 2) HV+LV (HL), 3) HV+LV followed by HV+LV with no change in polarity (HLHL unidirectional), 4) HV+LV followed by HV+LV with opposite polarity (HLHL bidirectional), 5) HV+LV followed by LV with opposite polarity (HLL bidirectional). Results: HLL bidirectional sequence showed the best result with less interindividual variability and extended expression period. With the exception of repeated high voltage sequences, EP parameters were not likely to induce cell injury or inflammation. Conclusion: HLL bidirectional electroporative gene delivery produces high transfection rates with limited tissue trauma. Significance: Bidirectional EP provides a safe and highly efficient method for therapeutic gene delivery into skeletal muscle.

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JO - Otolaryngology - Head and Neck Surgery

JF - Otolaryngology - Head and Neck Surgery

IS - 2

ER -

High efficiency gene delivery into laryngeal muscle with bidirectional electroporation

Abstract

Funding

ASJC Scopus subject areas

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