Human glycoprotein hormone production in human-human and human-mouse somatic cell hybrids

Mary R. Bordelon; H. G. Coon; P. O. Kohler

doi:10.1016/0014-4827(76)90267-6

Human glycoprotein hormone production in human-human and human-mouse somatic cell hybrids

Mary R. Bordelon
, H. G. Coon
, P. O. Kohler

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The production of both protein and steroid hormones was studied utilizing somatic cell hybrids formed with human choriocarcinoma cells. The human JEG-3 cell line produced the species and organ-specific hormone human chorionic gonadotropin (hCG), the steroid hormone progesterone, and converted 19-carbon steroids to estrogens. Hybrids formed with human VA-2 cells, mouse Cl 1D cells and mouse 3T3-4EF cells had detectable hCG synthesis in 20 of 41 total clones. There was no detectable progesterone or 19-carbon aromatization to estrogens in any hybrids. These data demonstrate that the differentiated function of human protein hormone production can be retained in inter- and intra-specific somatic cell hybrids. These results also suggest that protein hormone production can occur independently of steroid production in these cells of placental origin.

Original language	English (US)
Pages (from-to)	303-310
Number of pages	8
Journal	Experimental Cell Research
Volume	103
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(76)90267-6
State	Published - Dec 1976
Externally published	Yes

Funding

This work was supportedb y NIH Grant AM-17307 and PostdoctoralF ellowship CA01935t o Marf R. Bor-delon.

Funders	Funder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	AM-17307

ASJC Scopus subject areas

Cell Biology

Access to Document

10.1016/0014-4827(76)90267-6

Cite this

@article{c25ab021caf74135a201e1d074755e24,

title = "Human glycoprotein hormone production in human-human and human-mouse somatic cell hybrids",

abstract = "The production of both protein and steroid hormones was studied utilizing somatic cell hybrids formed with human choriocarcinoma cells. The human JEG-3 cell line produced the species and organ-specific hormone human chorionic gonadotropin (hCG), the steroid hormone progesterone, and converted 19-carbon steroids to estrogens. Hybrids formed with human VA-2 cells, mouse Cl 1D cells and mouse 3T3-4EF cells had detectable hCG synthesis in 20 of 41 total clones. There was no detectable progesterone or 19-carbon aromatization to estrogens in any hybrids. These data demonstrate that the differentiated function of human protein hormone production can be retained in inter- and intra-specific somatic cell hybrids. These results also suggest that protein hormone production can occur independently of steroid production in these cells of placental origin.",

author = "Bordelon, \{Mary R.\} and Coon, \{H. G.\} and Kohler, \{P. O.\}",

note = "Funding Information: This work was supportedb y NIH Grant AM-17307 and PostdoctoralF ellowship CA01935t o Marf R. Bor-delon.",

year = "1976",

month = dec,

doi = "10.1016/0014-4827(76)90267-6",

language = "English (US)",

volume = "103",

pages = "303--310",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Human glycoprotein hormone production in human-human and human-mouse somatic cell hybrids

AU - Bordelon, Mary R.

AU - Coon, H. G.

AU - Kohler, P. O.

N1 - Funding Information: This work was supportedb y NIH Grant AM-17307 and PostdoctoralF ellowship CA01935t o Marf R. Bor-delon.

PY - 1976/12

Y1 - 1976/12

N2 - The production of both protein and steroid hormones was studied utilizing somatic cell hybrids formed with human choriocarcinoma cells. The human JEG-3 cell line produced the species and organ-specific hormone human chorionic gonadotropin (hCG), the steroid hormone progesterone, and converted 19-carbon steroids to estrogens. Hybrids formed with human VA-2 cells, mouse Cl 1D cells and mouse 3T3-4EF cells had detectable hCG synthesis in 20 of 41 total clones. There was no detectable progesterone or 19-carbon aromatization to estrogens in any hybrids. These data demonstrate that the differentiated function of human protein hormone production can be retained in inter- and intra-specific somatic cell hybrids. These results also suggest that protein hormone production can occur independently of steroid production in these cells of placental origin.

AB - The production of both protein and steroid hormones was studied utilizing somatic cell hybrids formed with human choriocarcinoma cells. The human JEG-3 cell line produced the species and organ-specific hormone human chorionic gonadotropin (hCG), the steroid hormone progesterone, and converted 19-carbon steroids to estrogens. Hybrids formed with human VA-2 cells, mouse Cl 1D cells and mouse 3T3-4EF cells had detectable hCG synthesis in 20 of 41 total clones. There was no detectable progesterone or 19-carbon aromatization to estrogens in any hybrids. These data demonstrate that the differentiated function of human protein hormone production can be retained in inter- and intra-specific somatic cell hybrids. These results also suggest that protein hormone production can occur independently of steroid production in these cells of placental origin.

UR - https://www.scopus.com/pages/publications/0017029518

UR - https://www.scopus.com/pages/publications/0017029518#tab=citedBy

U2 - 10.1016/0014-4827(76)90267-6

DO - 10.1016/0014-4827(76)90267-6

M3 - Article

C2 - 137122

AN - SCOPUS:0017029518

SN - 0014-4827

VL - 103

SP - 303

EP - 310

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

Human glycoprotein hormone production in human-human and human-mouse somatic cell hybrids

Abstract

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this