Human Milk Fatty Acid Composition: Comparison of Novel Dried Milk Spot Versus Standard Liquid Extraction Methods

Michael C. Rudolph; Bridget E. Young; Kristina Harris Jackson; Nancy F. Krebs; William S. Harris; Paul S. MacLean

doi:10.1007/s10911-016-9365-4

Human Milk Fatty Acid Composition: Comparison of Novel Dried Milk Spot Versus Standard Liquid Extraction Methods

Michael C. Rudolph
, Bridget E. Young
, Kristina Harris Jackson
, Nancy F. Krebs
, William S. Harris
, Paul S. MacLean

Academic Affairs

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Accurate assessment of the long chain polyunsaturated fatty acid (LC-PUFA) content of human milk (HM) provides a powerful means to evaluate the FA nutrient status of breastfed infants. The conventional standard for FA composition analysis of HM is liquid extraction, trans-methylation, and analyte detection resolved by gas chromatography. This standard approach requires fresh or frozen samples, storage in deep freeze, organic solvents, and specialized equipment in processing and analysis. Further, HM collection is often impractical for many studies in the free living environment, particularly for studies in developing countries. In the present study, we compare a novel and more practical approach to sample collection and processing that involves the spotting and drying ~50 μL of HM on a specialized paper stored and transported at ambient temperatures until analysis. Deming regression indicated the two methods aligned very well for all LC-PUFA and the abundant HM FA. Additionally, strong correlations (r > 0.85) were observed for DHA, ARA, EPA, linoleic (LA), and alpha-linolenic acids (ALA), which are of particular interest to the health of the developing infant. Taken together, our data suggest this more practical and inexpensive method of collection, storage, and transport of HM milk samples could dramatically facilitate studies of HM, as well as understanding its lipid composition influences on human health and development.

Original language	English (US)
Pages (from-to)	131-138
Number of pages	8
Journal	Journal of Mammary Gland Biology and Neoplasia
Volume	21
Issue number	3-4
DOIs	https://doi.org/10.1007/s10911-016-9365-4
State	Published - Dec 1 2016

Funding

The authors would like to express our sincere appreciation to all of our study participants and those who provided critical reading. MCR is supported by Building Interdisciplinary Careers in Women’s Health NIH K-12 HD057022, NICHD T32-HD007186 training grant, and Nutrition and Obesity Research Center pilot funding P30-DK048520. BEY by NICHD F32-HD0978068, T32-DK007658-21, Thrasher Research Fund Early Career Award, and Center for Women’s Health Research. NFK by K24-DK083772. PSM by P01-HD038129, P50 HD073063, R01 CA164166, and R01-HD075285. Lipid mass spectrometry supported by Dr. Robert Murphy and NIH/NCATS Colorado CTSA Grant UL1 TR001082. OmegaQuant internally funded the dried milk spot analysis. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Funders	Funder number
Building Interdisciplinary Careers in Women’s Health NIH	K-12 HD057022
Center for Women’s Health Research	K24-DK083772
New York Nutrition and Obesity Research Center	P30-DK048520, T32-DK007658-21, F32-HD0978068
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health
National Institute of Diabetes and Digestive and Kidney Diseases	T32DK007658
Thrasher Research Fund
National Center for Advancing Translational Sciences	UL1 TR001082
Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health	T32-HD007186

Keywords

Fatty acid composition
Human milk
Long chain polyunsaturated fatty acids
Mass spectrometry
Omega-3

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1007/s10911-016-9365-4

Cite this

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title = "Human Milk Fatty Acid Composition: Comparison of Novel Dried Milk Spot Versus Standard Liquid Extraction Methods",

abstract = "Accurate assessment of the long chain polyunsaturated fatty acid (LC-PUFA) content of human milk (HM) provides a powerful means to evaluate the FA nutrient status of breastfed infants. The conventional standard for FA composition analysis of HM is liquid extraction, trans-methylation, and analyte detection resolved by gas chromatography. This standard approach requires fresh or frozen samples, storage in deep freeze, organic solvents, and specialized equipment in processing and analysis. Further, HM collection is often impractical for many studies in the free living environment, particularly for studies in developing countries. In the present study, we compare a novel and more practical approach to sample collection and processing that involves the spotting and drying \textasciitilde{}50 μL of HM on a specialized paper stored and transported at ambient temperatures until analysis. Deming regression indicated the two methods aligned very well for all LC-PUFA and the abundant HM FA. Additionally, strong correlations (r > 0.85) were observed for DHA, ARA, EPA, linoleic (LA), and alpha-linolenic acids (ALA), which are of particular interest to the health of the developing infant. Taken together, our data suggest this more practical and inexpensive method of collection, storage, and transport of HM milk samples could dramatically facilitate studies of HM, as well as understanding its lipid composition influences on human health and development.",

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Human Milk Fatty Acid Composition: Comparison of Novel Dried Milk Spot Versus Standard Liquid Extraction Methods

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