TY - JOUR
T1 - Human thymus-independent antibody response in vitro
T2 - III. Precursor frequencies and fine specificity of human B cells stimulated by TNP-Ba in the presence and absence of Con A
AU - Golding, Basil
AU - Rittenberg, Marvin B.
N1 - Funding Information:
Dr. B. Golding is a postdoctoral fellow of the Arthritis Foundation. This work was supported by National Institutes of Health Grant AI 14985 and Basic Research Grant I-633 from the March of Dimes Birth Defects Foundation. We also thank Drs. H. Golding, S. P. Chang, I. Todd, J. Berzofsky, A. Muchmore and R. Yarchoan; and R. Jones, F. Makowski, J-P Li, and L. Jobe for critical comments and discussion of the manuscript; and K. L. Pratt for expert technical assistance.
PY - 1984/5
Y1 - 1984/5
N2 - Previously, we reported that human B lymphocytes can be stimulated by trinitrophenylated Brucella abortus (TNP-Ba) in vitro to generate T-independent (TI) hapten specific plaque-forming cells (PFC). Furthermore we showed that addition of Con A on Day 3 of culture enhanced the anti-TNP response. In this report the characteristics of the anti-TNP PFC responses elicited by TNP-Ba in the presence or absence of Con A were further defined by estimating precursor frequencies and clone sizes, and by assessing the diversity of the anti-TNP-PFC response using hapten inhibition profiles. Addition of Con A to TNP-Ba-stimulated cultures was associated with a 2.3- to 3.6-fold increase in anti-TNP precursors and was also accompanied by a more vigorous clonal expansion. Fine specificity analysis of anti-TNP PFC revealed that Con A addition resulted in PFC with unique hapten inhibition profiles in that they were less inhibitable by TNP-EACA, TNP-lysine, and DNP-lysine but more inhibitable by DNP-glycine when compared to PFC generated by TNP-Ba alone. These findings suggest that at least some of the additional precursors recruited in the presence of Con A are qualitatively distinct from those activated by TNP-Ba alone since they express different V region gene products.
AB - Previously, we reported that human B lymphocytes can be stimulated by trinitrophenylated Brucella abortus (TNP-Ba) in vitro to generate T-independent (TI) hapten specific plaque-forming cells (PFC). Furthermore we showed that addition of Con A on Day 3 of culture enhanced the anti-TNP response. In this report the characteristics of the anti-TNP PFC responses elicited by TNP-Ba in the presence or absence of Con A were further defined by estimating precursor frequencies and clone sizes, and by assessing the diversity of the anti-TNP-PFC response using hapten inhibition profiles. Addition of Con A to TNP-Ba-stimulated cultures was associated with a 2.3- to 3.6-fold increase in anti-TNP precursors and was also accompanied by a more vigorous clonal expansion. Fine specificity analysis of anti-TNP PFC revealed that Con A addition resulted in PFC with unique hapten inhibition profiles in that they were less inhibitable by TNP-EACA, TNP-lysine, and DNP-lysine but more inhibitable by DNP-glycine when compared to PFC generated by TNP-Ba alone. These findings suggest that at least some of the additional precursors recruited in the presence of Con A are qualitatively distinct from those activated by TNP-Ba alone since they express different V region gene products.
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U2 - 10.1016/0008-8749(84)90245-4
DO - 10.1016/0008-8749(84)90245-4
M3 - Article
C2 - 6424950
AN - SCOPUS:0021354518
SN - 0008-8749
VL - 85
SP - 309
EP - 317
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -