Human transferrin receptor: Expression of the receptor is assigned to chromosome 3

C. A. Enns, H. A. Suomalainen, J. E. Gebhardt, J. Schröder, H. H. Sussman

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Human chromosome 3 has been identified as responsible for expression of the transferrin receptor in mouse-human lymphocyte hybrids. The receptor was detected by immunoprecipitation with anti-human receptor antibody of 125I-labeled cells. This method also detected a similar 94,000-dalton protein in mouse cells. A radioimmunoassay developed for the human transferrin receptor measured 10% crossreactivity with the mouse protein. The two proteins were distinguished by NaDodSo4/polyacrylamide gel patterns of partial proteolytic digests of the immunoprecipitated proteins. Mouse-human hybrids were generated by fusing a mouse thymoma (BW5147) cell line to either concanavalin A- or pokeweed mitogen-activated human peripheral blood lymphocytes or a mouse myeloma (NS-1) to uncultured human peripheral blood lymphocytes. Each hybrid was karyotyped with respect to both mouse and human chromosomes. In every case, expression of the human transferrin receptor correlated only with human chromosome 3.

Original languageEnglish (US)
Pages (from-to)3241-3245
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number10 I
StatePublished - 1982
Externally publishedYes

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Human transferrin receptor: Expression of the receptor is assigned to chromosome 3'. Together they form a unique fingerprint.

Cite this