Hypertonic saline injection model of experimental glaucoma in rats

John C. Morrison, Elaine C. Johnson, William O. Cepurna

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Scopus citations


A reliable method of creating chronic elevation of intraocular pressure (IOP) in rodents is an important tool in reproducing and studying the mechanisms of optic nerve injury that occur in glaucoma. In addition, such a model could provide a valuable method for testing potential neuroprotective treatments. This paper outlines the basic methods for producing obstruction of aqueous humor outflow and IOP elevation by injecting hypertonic saline (a sclerosant) into the aqueous outflow pathway. This is one of several rodent glaucoma models in use today. In this method, a plastic ring is placed around the equator of the eye to restrict injected saline to the limbus. By inserting a small glass microneedle in an aqueous outflow vein in the episclera and injecting hypertonic saline toward the limbus, the saline is forced into Schlemm’s canal and across the trabecular meshwork. The resultant inflammation and scarring of the anterior chamber angle occurs gradually, resulting in a rise in IOP after approximately 1 week. This article will describe the equipment necessary for producing this model and the steps of the technique itself.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages11
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Aqueous humor
  • Brown Norway rat
  • Chronic angle closure
  • Episcleral veins
  • Glaucoma
  • Hypertonic saline
  • Intraocular pressure
  • Optic nerve

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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