Density gradient ultracentrifugation and high resolution polyacrylamide gel electrophoresis (Lipoprint LDL-system) were used to examine the discrete LDL subfraction patterns in normal and hyperlipidemic patients. Two new programs for LDL and VLDL subfractions were elaborated and a new method of evaluation for cholesterol content in LDL-subfractions, based on area distribution ratio of densitograms without density gradient ultracentrifugation, was created. The predominance of cholesterol and triglycerides in dense LDL3-subclass (d-1,048-1,063 g/ml) under hypercholesterolemia and hypertriglyceridemia was found. Lipoprint LDL-electrophoresis method was developed for the characteristic of lipemic state of organism and detection and quantitation of plasma LDL subfractions.
|Original language||English (US)|
|Number of pages||6|
|Journal||Ukrainskii biokhimicheskii zhurnal|
|State||Published - 1995|
ASJC Scopus subject areas