Identification and Characterization of the Hamster Polyomavirus Middle T Antigen

Hamster polyomavirus (HaPV) is associated with lymphoid and hair follicle tumors in Syrian hamsters. The early region of HaPV has the potential to encode three polypeptides (which are related to the mouse polyomavirus early proteins) and can transform fibroblasts in vitro. We identified the HaPV middle T antigen (HamT) as a 45-kDa protein. Like its murine counterpart, HamT was associated with serine/threonine phosphatase, phosphatidylinositol-3 kinase, and protein tyrosine kinase activities. However, whereas mouse middle T antigen associates predominantly with pp60^c-src and pp62^c-yes, HamT was associated with a different tyrosine kinase, p59^fyn. The ability of HaPV to cause lymphoid tumors may therefore reside in its ability to associate with p59^fyn, a potentially important tyrosine kinase in lymphocytes.