Identification of cDNA clones of the mouse neural cell adhesion molecule L1

Roland Tacke; Marion Moos; David B. Teplow; Klaus Früh; Herta Scherer; Alfred Bach; Melitta Schachner

doi:10.1016/0304-3940(87)90176-5

Identification of cDNA clones of the mouse neural cell adhesion molecule L1

Roland Tacke, Marion Moos, David B. Teplow, Klaus Früh, Herta Scherer, Alfred Bach, Melitta Schachner

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Two cDNA clones of the neural cell adhesion molecule L1 (M_r 200,000) were isolated using λgt10 and λgt11 libraries constructed from postnatal day 8 mouse brain poly(A)⁺ RNA. Clone K21 was selected and identified using immunoaffinity purified polyclonal antibodies. It was then used to isolate a secondary clone (K21-1), which hybridized with an oligonucleotide probe synthesized by reverse translation of the aminoterminal sequence of the 80 kDa carboxyterminal proteolytic fragment of L1. Blot hybridization analysis indicated that L1 is encoded by a single gene and transcribed by a single 6 kb mRNA which is present only in cells or tissues known to express L1.

Original language	English (US)
Pages (from-to)	89-94
Number of pages	6
Journal	Neuroscience Letters
Volume	82
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(87)90176-5
State	Published - Nov 10 1987
Externally published	Yes

Keywords

Cell adhesion
L1
Mouse
Nervous system
cDNA

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0304-3940(87)90176-5

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title = "Identification of cDNA clones of the mouse neural cell adhesion molecule L1",

abstract = "Two cDNA clones of the neural cell adhesion molecule L1 (Mr 200,000) were isolated using λgt10 and λgt11 libraries constructed from postnatal day 8 mouse brain poly(A)+ RNA. Clone K21 was selected and identified using immunoaffinity purified polyclonal antibodies. It was then used to isolate a secondary clone (K21-1), which hybridized with an oligonucleotide probe synthesized by reverse translation of the aminoterminal sequence of the 80 kDa carboxyterminal proteolytic fragment of L1. Blot hybridization analysis indicated that L1 is encoded by a single gene and transcribed by a single 6 kb mRNA which is present only in cells or tissues known to express L1.",

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AU - Tacke, Roland

AU - Moos, Marion

AU - Teplow, David B.

AU - Früh, Klaus

AU - Scherer, Herta

AU - Bach, Alfred

AU - Schachner, Melitta

PY - 1987/11/10

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AB - Two cDNA clones of the neural cell adhesion molecule L1 (Mr 200,000) were isolated using λgt10 and λgt11 libraries constructed from postnatal day 8 mouse brain poly(A)+ RNA. Clone K21 was selected and identified using immunoaffinity purified polyclonal antibodies. It was then used to isolate a secondary clone (K21-1), which hybridized with an oligonucleotide probe synthesized by reverse translation of the aminoterminal sequence of the 80 kDa carboxyterminal proteolytic fragment of L1. Blot hybridization analysis indicated that L1 is encoded by a single gene and transcribed by a single 6 kb mRNA which is present only in cells or tissues known to express L1.

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Identification of cDNA clones of the mouse neural cell adhesion molecule L1

Abstract

Keywords

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