Identification of receptors for insulin-like growth factor II in two insulin-like growth factor II producing cell lines

P. D.K. Lee; D. Hodges; R. L. Hintz; J. H. Wyche; R. G. Rosenfeld

doi:10.1016/S0006-291X(86)80461-2

Identification of receptors for insulin-like growth factor II in two insulin-like growth factor II producing cell lines

P. D.K. Lee
, D. Hodges
, R. L. Hintz
, J. H. Wyche
, R. G. Rosenfeld

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Specific high affinity membrane receptor(s) for insulin-like growth factor II have been characterized in two cell lines which produce this hormone and have the ability to proliferate in serum-free media. These receptor(s) have no affinity for either insulin or biosynthetic insulin-like growth factor I. Affinity cross-linking and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed an apparent Mr of 250K which does not change with disulfide bond reduction. Our findings are consistent with an autocrine function for insulin-like growth factor II and indicate that these continuous cell lines may provide unique systems for furthur investigations of this hormone and its receptor.

Original language	English (US)
Pages (from-to)	595-600
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	134
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(86)80461-2
State	Published - Jan 29 1986
Externally published	Yes

Funding

Acknowledgements: We thank Bonita Baker for performance of the IGF-II assays. Dr. Lee is supported by a fellowship grant from the Juvenile Diabetes Foundation International. Dm. Rosenfeld is the recipient of a Research Career Development Award from the NIH, This work was supported by NIH grants AM28229 (RGR) and AM24085 (RLH).

Funders	Funder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	AM28229, AM24085
Juvenile Diabetes Research Foundation International

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(86)80461-2

Cite this

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title = "Identification of receptors for insulin-like growth factor II in two insulin-like growth factor II producing cell lines",

abstract = "Specific high affinity membrane receptor(s) for insulin-like growth factor II have been characterized in two cell lines which produce this hormone and have the ability to proliferate in serum-free media. These receptor(s) have no affinity for either insulin or biosynthetic insulin-like growth factor I. Affinity cross-linking and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed an apparent Mr of 250K which does not change with disulfide bond reduction. Our findings are consistent with an autocrine function for insulin-like growth factor II and indicate that these continuous cell lines may provide unique systems for furthur investigations of this hormone and its receptor.",

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note = "Funding Information: Acknowledgements: We thank Bonita Baker for performance of the IGF-II assays. Dr. Lee is supported by a fellowship grant from the Juvenile Diabetes Foundation International. Dm. Rosenfeld is the recipient of a Research Career Development Award from the NIH, This work was supported by NIH grants AM28229 (RGR) and AM24085 (RLH).",

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doi = "10.1016/S0006-291X(86)80461-2",

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AU - Hodges, D.

AU - Hintz, R. L.

AU - Wyche, J. H.

AU - Rosenfeld, R. G.

N1 - Funding Information: Acknowledgements: We thank Bonita Baker for performance of the IGF-II assays. Dr. Lee is supported by a fellowship grant from the Juvenile Diabetes Foundation International. Dm. Rosenfeld is the recipient of a Research Career Development Award from the NIH, This work was supported by NIH grants AM28229 (RGR) and AM24085 (RLH).

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N2 - Specific high affinity membrane receptor(s) for insulin-like growth factor II have been characterized in two cell lines which produce this hormone and have the ability to proliferate in serum-free media. These receptor(s) have no affinity for either insulin or biosynthetic insulin-like growth factor I. Affinity cross-linking and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed an apparent Mr of 250K which does not change with disulfide bond reduction. Our findings are consistent with an autocrine function for insulin-like growth factor II and indicate that these continuous cell lines may provide unique systems for furthur investigations of this hormone and its receptor.

AB - Specific high affinity membrane receptor(s) for insulin-like growth factor II have been characterized in two cell lines which produce this hormone and have the ability to proliferate in serum-free media. These receptor(s) have no affinity for either insulin or biosynthetic insulin-like growth factor I. Affinity cross-linking and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed an apparent Mr of 250K which does not change with disulfide bond reduction. Our findings are consistent with an autocrine function for insulin-like growth factor II and indicate that these continuous cell lines may provide unique systems for furthur investigations of this hormone and its receptor.

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Identification of receptors for insulin-like growth factor II in two insulin-like growth factor II producing cell lines

Abstract

Funding

ASJC Scopus subject areas

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