IL-7 enhances Ag-specific human T cell response by increasing expression of IL-2R α and γ chains

Yuan K. Chou; Dennis N. Bourdette; David Barnes; Thomas P. Finn; Susan Murray; Laura Unsicker; Ian Robey; Ruth H. Whitham; Abigail C. Buenafe; Mark Allegretta; Halina Offner; Arthur A. Vandenbark

doi:10.1016/S0165-5728(99)00002-8

IL-7 enhances Ag-specific human T cell response by increasing expression of IL-2R α and γ chains

Yuan K. Chou
, Dennis N. Bourdette
, David Barnes
, Thomas P. Finn
, Susan Murray
, Laura Unsicker
, Ian Robey
, Ruth H. Whitham
, Abigail C. Buenafe
, Mark Allegretta
, Halina Offner
, Arthur A. Vandenbark

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Interleukin-7 has demonstrated potent enhancing effects on the growth and differentiation of several immature celt types, including thymocytes, and on survival of resting and antigen activated T cells. In this study, we evaluated the effects of IL-7 on post-thymic antigen-specific T cells from human blood. IL-7 was found to enhance proliferation responses and IFN-γ secretion of myelin or recall Ag-specific Th1 cells through the selective up- regulation of the IL-2Rα and γ but not β chains in both an Ag-dependent and Ag-independent manner, but did not affect monocytes, B cells, or NK cells. These functions of IL-7 enhanced the detection of Th1 but not Th2 cell frequency by > 2.5 fold, and promoted selection of Ag-specific Th1 cells by the limiting dilution method. Moreover, IL-7 pretreatment conferred increased resistance of CD4 + T cells to CD8 + cell lysis. These studies demonstrate that IL-7 promotes the growth and survival of circulating Ag-specific human Th1 cells through a mechanism that probably involves the γc common receptor for IL-2 family members that includes IL-7.

Original language	English (US)
Pages (from-to)	101-111
Number of pages	11
Journal	Journal of Neuroimmunology
Volume	96
Issue number	1
DOIs	https://doi.org/10.1016/S0165-5728(99)00002-8
State	Published - Apr 1 1999

Funding

The authors thank Shelley Cooper for arranging patient visits and drawing blood, Eva Niehaus for assistance in preparation of the manuscript, and Dr. Raymond Goodwin of Immunex for reviewing the manuscript. This work was supported by the Department of Veterans Affairs, Grants NS23221 and NS23444 from the National Institutes of Health, the Nancy Davis Center Without Walls, and private donors.

Funders	Funder number
Nancy Davis Center Without Walls Foundation
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health
National Institute of Neurological Disorders and Stroke	R01NS023444
U.S. Department of Veterans Affairs	NS23221

Keywords

Human T cells
IL-2R
IL-7
Survival factor

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

Access to Document

10.1016/S0165-5728(99)00002-8

Cite this

Chou, Y. K., Bourdette, D. N., Barnes, D., Finn, T. P., Murray, S., Unsicker, L., Robey, I., Whitham, R. H., Buenafe, A. C., Allegretta, M., Offner, H., & Vandenbark, A. A. (1999). IL-7 enhances Ag-specific human T cell response by increasing expression of IL-2R α and γ chains. Journal of Neuroimmunology, 96(1), 101-111. https://doi.org/10.1016/S0165-5728(99)00002-8

@article{6b84e82a23f445c7ba2c93648bc9e790,

title = "IL-7 enhances Ag-specific human T cell response by increasing expression of IL-2R α and γ chains",

abstract = "Interleukin-7 has demonstrated potent enhancing effects on the growth and differentiation of several immature celt types, including thymocytes, and on survival of resting and antigen activated T cells. In this study, we evaluated the effects of IL-7 on post-thymic antigen-specific T cells from human blood. IL-7 was found to enhance proliferation responses and IFN-γ secretion of myelin or recall Ag-specific Th1 cells through the selective up- regulation of the IL-2Rα and γ but not β chains in both an Ag-dependent and Ag-independent manner, but did not affect monocytes, B cells, or NK cells. These functions of IL-7 enhanced the detection of Th1 but not Th2 cell frequency by > 2.5 fold, and promoted selection of Ag-specific Th1 cells by the limiting dilution method. Moreover, IL-7 pretreatment conferred increased resistance of CD4 + T cells to CD8 + cell lysis. These studies demonstrate that IL-7 promotes the growth and survival of circulating Ag-specific human Th1 cells through a mechanism that probably involves the γc common receptor for IL-2 family members that includes IL-7.",

keywords = "Human T cells, IL-2R, IL-7, Survival factor",

author = "Chou, \{Yuan K.\} and Bourdette, \{Dennis N.\} and David Barnes and Finn, \{Thomas P.\} and Susan Murray and Laura Unsicker and Ian Robey and Whitham, \{Ruth H.\} and Buenafe, \{Abigail C.\} and Mark Allegretta and Halina Offner and Vandenbark, \{Arthur A.\}",

note = "Funding Information: The authors thank Shelley Cooper for arranging patient visits and drawing blood, Eva Niehaus for assistance in preparation of the manuscript, and Dr. Raymond Goodwin of Immunex for reviewing the manuscript. This work was supported by the Department of Veterans Affairs, Grants NS23221 and NS23444 from the National Institutes of Health, the Nancy Davis Center Without Walls, and private donors.",

year = "1999",

month = apr,

day = "1",

doi = "10.1016/S0165-5728(99)00002-8",

language = "English (US)",

volume = "96",

pages = "101--111",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier BV",

number = "1",

}

TY - JOUR

T1 - IL-7 enhances Ag-specific human T cell response by increasing expression of IL-2R α and γ chains

AU - Chou, Yuan K.

AU - Bourdette, Dennis N.

AU - Barnes, David

AU - Finn, Thomas P.

AU - Murray, Susan

AU - Unsicker, Laura

AU - Robey, Ian

AU - Whitham, Ruth H.

AU - Buenafe, Abigail C.

AU - Allegretta, Mark

AU - Offner, Halina

AU - Vandenbark, Arthur A.

N1 - Funding Information: The authors thank Shelley Cooper for arranging patient visits and drawing blood, Eva Niehaus for assistance in preparation of the manuscript, and Dr. Raymond Goodwin of Immunex for reviewing the manuscript. This work was supported by the Department of Veterans Affairs, Grants NS23221 and NS23444 from the National Institutes of Health, the Nancy Davis Center Without Walls, and private donors.

PY - 1999/4/1

Y1 - 1999/4/1

N2 - Interleukin-7 has demonstrated potent enhancing effects on the growth and differentiation of several immature celt types, including thymocytes, and on survival of resting and antigen activated T cells. In this study, we evaluated the effects of IL-7 on post-thymic antigen-specific T cells from human blood. IL-7 was found to enhance proliferation responses and IFN-γ secretion of myelin or recall Ag-specific Th1 cells through the selective up- regulation of the IL-2Rα and γ but not β chains in both an Ag-dependent and Ag-independent manner, but did not affect monocytes, B cells, or NK cells. These functions of IL-7 enhanced the detection of Th1 but not Th2 cell frequency by > 2.5 fold, and promoted selection of Ag-specific Th1 cells by the limiting dilution method. Moreover, IL-7 pretreatment conferred increased resistance of CD4 + T cells to CD8 + cell lysis. These studies demonstrate that IL-7 promotes the growth and survival of circulating Ag-specific human Th1 cells through a mechanism that probably involves the γc common receptor for IL-2 family members that includes IL-7.

AB - Interleukin-7 has demonstrated potent enhancing effects on the growth and differentiation of several immature celt types, including thymocytes, and on survival of resting and antigen activated T cells. In this study, we evaluated the effects of IL-7 on post-thymic antigen-specific T cells from human blood. IL-7 was found to enhance proliferation responses and IFN-γ secretion of myelin or recall Ag-specific Th1 cells through the selective up- regulation of the IL-2Rα and γ but not β chains in both an Ag-dependent and Ag-independent manner, but did not affect monocytes, B cells, or NK cells. These functions of IL-7 enhanced the detection of Th1 but not Th2 cell frequency by > 2.5 fold, and promoted selection of Ag-specific Th1 cells by the limiting dilution method. Moreover, IL-7 pretreatment conferred increased resistance of CD4 + T cells to CD8 + cell lysis. These studies demonstrate that IL-7 promotes the growth and survival of circulating Ag-specific human Th1 cells through a mechanism that probably involves the γc common receptor for IL-2 family members that includes IL-7.

KW - Human T cells

KW - IL-2R

KW - IL-7

KW - Survival factor

UR - https://www.scopus.com/pages/publications/0033119580

UR - https://www.scopus.com/pages/publications/0033119580#tab=citedBy

U2 - 10.1016/S0165-5728(99)00002-8

DO - 10.1016/S0165-5728(99)00002-8

M3 - Article

C2 - 10227429

AN - SCOPUS:0033119580

SN - 0165-5728

VL - 96

SP - 101

EP - 111

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1

ER -

IL-7 enhances Ag-specific human T cell response by increasing expression of IL-2R α and γ chains

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this