TY - JOUR
T1 - Immediate-early gene responses in the avian song control system
T2 - cloning and expression analysis of the canary c-jun cDNA
AU - Nastiuk, Kent L.
AU - Mello, Claudio V.
AU - George, Julia M.
AU - Clayton, David F.
N1 - Funding Information:
This work was supported by grants from the Whitehall Foundation and the NIH (NS-25742). We gratefully acknowledge the contributions of Fernando Not-tebohm and the many members of his laboratory who have interacted with us.
PY - 1994/12
Y1 - 1994/12
N2 - Previous studies have shown that song presentation results in a rapid rise in mRNA levels for the ZENK gene (the avian homologue of zif-268, Egr-1, NGFI-A, and Krox-24) in specific parts of the songbird forebrain. Metrazole-induced seizures also cause an increase in ZENK mRNA, even more widely throughout the telencephalon. Surprisingly, however, little or no ZENK induction by either stimulus was observed in several forebrain areas involved in auditory processing and song production. To learn whether this pattern of regulation is specific to ZENK, we examined the response of another 'immediate-early' gene, c-jun. Here we first describe the identification, cloning and sequence analysis of a canary cDNA encoding c-jun. Then, by in situ hybridization we show that c-jun is also induced by song or seizure, and in a pattern mostly similar to ZENK. As with ZENK, no induction of c-jun is observed in the androgen receptor-containing song nuclei or within the primary thalamo-recipient auditory area of the forebrain. Thus common immediate early gene responses appear to be selectively uncoupled from physiological activation in these specific forebrain regions, which are also characterized by tight developmental, hormonal and seasonal regulation.
AB - Previous studies have shown that song presentation results in a rapid rise in mRNA levels for the ZENK gene (the avian homologue of zif-268, Egr-1, NGFI-A, and Krox-24) in specific parts of the songbird forebrain. Metrazole-induced seizures also cause an increase in ZENK mRNA, even more widely throughout the telencephalon. Surprisingly, however, little or no ZENK induction by either stimulus was observed in several forebrain areas involved in auditory processing and song production. To learn whether this pattern of regulation is specific to ZENK, we examined the response of another 'immediate-early' gene, c-jun. Here we first describe the identification, cloning and sequence analysis of a canary cDNA encoding c-jun. Then, by in situ hybridization we show that c-jun is also induced by song or seizure, and in a pattern mostly similar to ZENK. As with ZENK, no induction of c-jun is observed in the androgen receptor-containing song nuclei or within the primary thalamo-recipient auditory area of the forebrain. Thus common immediate early gene responses appear to be selectively uncoupled from physiological activation in these specific forebrain regions, which are also characterized by tight developmental, hormonal and seasonal regulation.
KW - Canary
KW - Immediate early gene
KW - Metrazole
KW - NGFI-A
KW - Songbird
KW - Zebra finch
KW - c-jun
KW - zif-268
UR - http://www.scopus.com/inward/record.url?scp=0028143435&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028143435&partnerID=8YFLogxK
U2 - 10.1016/0169-328X(94)90013-2
DO - 10.1016/0169-328X(94)90013-2
M3 - Article
C2 - 7898314
AN - SCOPUS:0028143435
SN - 0169-328X
VL - 27
SP - 299
EP - 309
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 2
ER -