Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces

Zheng Qu; Venkat Krishnamurthy; Carolyn A. Haller; Brent M. Dorr; Ulla M. Marzec; Sawan Hurst; Monica T. Hinds; Stephen R. Hanson; David R. Liu; Elliot L. Chaikof

doi:10.1002/adhm.201300110

Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces

Zheng Qu, Venkat Krishnamurthy, Carolyn A. Haller, Brent M. Dorr, Ulla M. Marzec, Sawan Hurst, Monica T. Hinds, Stephen R. Hanson, David R. Liu, Elliot L. Chaikof

Biomedical Engineering

Research output: Contribution to journal › Letter › peer-review

27 Scopus citations

Abstract

Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.

Original language	English (US)
Pages (from-to)	30-35
Number of pages	6
Journal	Advanced Healthcare Materials
Volume	3
Issue number	1
DOIs	https://doi.org/10.1002/adhm.201300110
State	Published - Jan 2014

Keywords

Antifouling
Biocompatible materials
Biomedical applications
Thrombomodulin
Thrombosis

ASJC Scopus subject areas

Biomaterials
Biomedical Engineering
Pharmaceutical Science

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10.1002/adhm.201300110

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title = "Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces",

abstract = "Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.",

keywords = "Antifouling, Biocompatible materials, Biomedical applications, Thrombomodulin, Thrombosis",

author = "Zheng Qu and Venkat Krishnamurthy and Haller, {Carolyn A.} and Dorr, {Brent M.} and Marzec, {Ulla M.} and Sawan Hurst and Hinds, {Monica T.} and Hanson, {Stephen R.} and Liu, {David R.} and Chaikof, {Elliot L.}",

year = "2014",

month = jan,

doi = "10.1002/adhm.201300110",

language = "English (US)",

volume = "3",

pages = "30--35",

journal = "Advanced healthcare materials",

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TY - JOUR

T1 - Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces

AU - Qu, Zheng

AU - Krishnamurthy, Venkat

AU - Haller, Carolyn A.

AU - Dorr, Brent M.

AU - Marzec, Ulla M.

AU - Hurst, Sawan

AU - Hinds, Monica T.

AU - Hanson, Stephen R.

AU - Liu, David R.

AU - Chaikof, Elliot L.

PY - 2014/1

Y1 - 2014/1

N2 - Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.

AB - Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.

KW - Antifouling

KW - Biocompatible materials

KW - Biomedical applications

KW - Thrombomodulin

KW - Thrombosis

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U2 - 10.1002/adhm.201300110

DO - 10.1002/adhm.201300110

M3 - Letter

C2 - 23788402

AN - SCOPUS:84891788106

SN - 2192-2640

VL - 3

SP - 30

EP - 35

JO - Advanced healthcare materials

JF - Advanced healthcare materials

IS - 1

ER -

Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces

Abstract

Keywords

ASJC Scopus subject areas

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