Immobilization of Actively Thromboresistant Assemblies on Sterile Blood-Contacting Surfaces

Zheng Qu, Venkat Krishnamurthy, Carolyn A. Haller, Brent M. Dorr, Ulla M. Marzec, Sawan Hurst, Monica T. Hinds, Stephen R. Hanson, David R. Liu, Elliot L. Chaikof

    Research output: Contribution to journalLetterpeer-review

    27 Scopus citations

    Abstract

    Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.

    Original languageEnglish (US)
    Pages (from-to)30-35
    Number of pages6
    JournalAdvanced Healthcare Materials
    Volume3
    Issue number1
    DOIs
    StatePublished - Jan 2014

    Keywords

    • Antifouling
    • Biocompatible materials
    • Biomedical applications
    • Thrombomodulin
    • Thrombosis

    ASJC Scopus subject areas

    • Biomaterials
    • Biomedical Engineering
    • Pharmaceutical Science

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