TY - JOUR
T1 - Immunological studies on the interaction of polyadenylic acid and human liver ribonuclease
AU - Neuwelt, Edward A.
AU - Gahl, William A.
AU - Levy, Carl C.
PY - 1976/12/20
Y1 - 1976/12/20
N2 - The effect of polyadenylic acid, a potent inhibitor of mammalian and bacterial RNAses, on the binding of human liver RNAse to its antibody was studied. To do this, a human liver RNAse antibody was immobilized on Sepharose 4B. Examination of the ability of the enzyme to bind to the immobilized anti-RNAse in the presence or absence of polyadenylic acid indicated that enzyme-antibody binding was more sensitive to the presence of polyadenylic acid than was enzyme activity. Furthermore, the effect of polyadenylic acid on enzyme-antibody binding was specific since neither polycytidylic acid nor polyuridylic acid had much effect on the antigenicity of the enzyme. The metal cation, Mg2+, and the polyamine, spermidine, but not putrescine, readily reversed the effects of polyadenylic acid on enzyme-antibody binding.
AB - The effect of polyadenylic acid, a potent inhibitor of mammalian and bacterial RNAses, on the binding of human liver RNAse to its antibody was studied. To do this, a human liver RNAse antibody was immobilized on Sepharose 4B. Examination of the ability of the enzyme to bind to the immobilized anti-RNAse in the presence or absence of polyadenylic acid indicated that enzyme-antibody binding was more sensitive to the presence of polyadenylic acid than was enzyme activity. Furthermore, the effect of polyadenylic acid on enzyme-antibody binding was specific since neither polycytidylic acid nor polyuridylic acid had much effect on the antigenicity of the enzyme. The metal cation, Mg2+, and the polyamine, spermidine, but not putrescine, readily reversed the effects of polyadenylic acid on enzyme-antibody binding.
UR - http://www.scopus.com/inward/record.url?scp=0017042861&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017042861&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(76)90205-9
DO - 10.1016/0006-291X(76)90205-9
M3 - Article
C2 - 15625858
AN - SCOPUS:0017042861
SN - 0006-291X
VL - 73
SP - 885
EP - 893
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -