Immunological studies with a monoclonal antibody suggest a different conformation of neurophysin in parvicellular neurons of rat hypothalamus

Gajanan Nilaver; Lawrence C. Rosenbaum; Hubert H.M. Van Tol; Eva Marie Shannon; Heidi M. Hagman; Earl A. Zimmerman; Edward A. Neuwelt

doi:10.1016/0006-8993(90)90841-X

Immunological studies with a monoclonal antibody suggest a different conformation of neurophysin in parvicellular neurons of rat hypothalamus

Gajanan Nilaver, Lawrence C. Rosenbaum, Hubert H.M. Van Tol, Eva Marie Shannon, Heidi M. Hagman, Earl A. Zimmerman, Edward A. Neuwelt

Neurology

Research output: Contribution to journal › Article › peer-review

Abstract

A monoclonal antibody (mAb L6) to a carcinoma surface antigen has previously been shown to recognize neurophysins (NP), proteins associated with oxytocin and vasopressin¹⁴. L6-reactivity in rat hypothalamus was confined to magnocellular neuronal systems. No staining was detected in parvicellular syprachiasmatic or paraventricular systems. mAb L6 immunoprecipitated vasopressin-neurophysin only under reducing conditions, and detected it in Western blots only after gel-renaturation and electroblotting in basic buffer. These findings suggest L6-reactivity to NP is conformation-sensitive, and imply NP expression in a unique configurational form in hypothalamic parvicellular systems.

Original language	English (US)
Pages (from-to)	302-308
Number of pages	7
Journal	Brain research
Volume	529
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(90)90841-X
State	Published - Oct 8 1990

Keywords

Hypothalamus
Immunohistochemistry
Immunoprecipitation
Monoclonal antibody
Neurophysin
Paraventricular nucleus
Suprachiasmatic nucleus
Supraoptic nucleus
Western blot

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(90)90841-X

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title = "Immunological studies with a monoclonal antibody suggest a different conformation of neurophysin in parvicellular neurons of rat hypothalamus",

abstract = "A monoclonal antibody (mAb L6) to a carcinoma surface antigen has previously been shown to recognize neurophysins (NP), proteins associated with oxytocin and vasopressin14. L6-reactivity in rat hypothalamus was confined to magnocellular neuronal systems. No staining was detected in parvicellular syprachiasmatic or paraventricular systems. mAb L6 immunoprecipitated vasopressin-neurophysin only under reducing conditions, and detected it in Western blots only after gel-renaturation and electroblotting in basic buffer. These findings suggest L6-reactivity to NP is conformation-sensitive, and imply NP expression in a unique configurational form in hypothalamic parvicellular systems.",

keywords = "Hypothalamus, Immunohistochemistry, Immunoprecipitation, Monoclonal antibody, Neurophysin, Paraventricular nucleus, Suprachiasmatic nucleus, Supraoptic nucleus, Western blot",

author = "Gajanan Nilaver and Rosenbaum, {Lawrence C.} and {Van Tol}, {Hubert H.M.} and Shannon, {Eva Marie} and Hagman, {Heidi M.} and Zimmerman, {Earl A.} and Neuwelt, {Edward A.}",

note = "Funding Information: We thank Drs. K.E. and I. HeUstr6m, Oncogen, Seattle, WA for their generous gift of mAb L6 and Pl.17. This work was supported by PHS Grant DK-37205 to E.A.Z. and G.N., NSF Grant BNS-8820600 to G.N., as well as the Veterans Administration and a National Cancer Institute Grant CA-31770 to E.A.N. and G.N. H.M.H. was supported by the N.L. Tartar Research Fellowship.",

year = "1990",

month = oct,

day = "8",

doi = "10.1016/0006-8993(90)90841-X",

language = "English (US)",

volume = "529",

pages = "302--308",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier",

number = "1-2",

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T1 - Immunological studies with a monoclonal antibody suggest a different conformation of neurophysin in parvicellular neurons of rat hypothalamus

AU - Nilaver, Gajanan

AU - Rosenbaum, Lawrence C.

AU - Van Tol, Hubert H.M.

AU - Shannon, Eva Marie

AU - Hagman, Heidi M.

AU - Zimmerman, Earl A.

AU - Neuwelt, Edward A.

N1 - Funding Information: We thank Drs. K.E. and I. HeUstr6m, Oncogen, Seattle, WA for their generous gift of mAb L6 and Pl.17. This work was supported by PHS Grant DK-37205 to E.A.Z. and G.N., NSF Grant BNS-8820600 to G.N., as well as the Veterans Administration and a National Cancer Institute Grant CA-31770 to E.A.N. and G.N. H.M.H. was supported by the N.L. Tartar Research Fellowship.

PY - 1990/10/8

Y1 - 1990/10/8

N2 - A monoclonal antibody (mAb L6) to a carcinoma surface antigen has previously been shown to recognize neurophysins (NP), proteins associated with oxytocin and vasopressin14. L6-reactivity in rat hypothalamus was confined to magnocellular neuronal systems. No staining was detected in parvicellular syprachiasmatic or paraventricular systems. mAb L6 immunoprecipitated vasopressin-neurophysin only under reducing conditions, and detected it in Western blots only after gel-renaturation and electroblotting in basic buffer. These findings suggest L6-reactivity to NP is conformation-sensitive, and imply NP expression in a unique configurational form in hypothalamic parvicellular systems.

AB - A monoclonal antibody (mAb L6) to a carcinoma surface antigen has previously been shown to recognize neurophysins (NP), proteins associated with oxytocin and vasopressin14. L6-reactivity in rat hypothalamus was confined to magnocellular neuronal systems. No staining was detected in parvicellular syprachiasmatic or paraventricular systems. mAb L6 immunoprecipitated vasopressin-neurophysin only under reducing conditions, and detected it in Western blots only after gel-renaturation and electroblotting in basic buffer. These findings suggest L6-reactivity to NP is conformation-sensitive, and imply NP expression in a unique configurational form in hypothalamic parvicellular systems.

KW - Hypothalamus

KW - Immunohistochemistry

KW - Immunoprecipitation

KW - Monoclonal antibody

KW - Neurophysin

KW - Paraventricular nucleus

KW - Suprachiasmatic nucleus

KW - Supraoptic nucleus

KW - Western blot

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Immunological studies with a monoclonal antibody suggest a different conformation of neurophysin in parvicellular neurons of rat hypothalamus

Abstract

Keywords

ASJC Scopus subject areas

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