Immunoreactivity assay for labeled anti-melanoma monoclonal antibodies

P. L. Beaumier; D. Neuzil; H. M. Yang; E. A. Noll; R. Kishore; J. F. Eary; K. A. Krohn; W. B. Nelp; K. E. Hellström; I. Hellström

Immunoreactivity assay for labeled anti-melanoma monoclonal antibodies

P. L. Beaumier, D. Neuzil, H. M. Yang, E. A. Noll, R. Kishore, J. F. Eary, K. A. Krohn, W. B. Nelp, K. E. Hellström, I. Hellström

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

A convenient, rapid, and reproducible assay was developed to evaluate the immunoreactivity of radiolabeled monoclonal antibodies against three different human melanoma-associated antigens, p97, a proteoglycan and a GD₃ ganglioside. A cloned melanoma cell line (M 2669 CL13) was selected as the target and, when fixed with paraformaldehyde, showed binding as good as or better than that obtained with live cells for the three antigens. Fixed cells retained good binding properties stored at 4°C for over 6 mo. This assay has general applicability to other antigen-antibody systems for testing chemically modified monoclonal antibodies or fragments during the development of a radiopharmaceutical or as a routine quality control measure for clinical agents.

Original language	English (US)
Pages (from-to)	824-828
Number of pages	5
Journal	Journal of Nuclear Medicine
Volume	27
Issue number	6
State	Published - 1986
Externally published	Yes

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

Cite this

@article{d799ce68ae074ee791be7145a83690ea,

title = "Immunoreactivity assay for labeled anti-melanoma monoclonal antibodies",

abstract = "A convenient, rapid, and reproducible assay was developed to evaluate the immunoreactivity of radiolabeled monoclonal antibodies against three different human melanoma-associated antigens, p97, a proteoglycan and a GD3 ganglioside. A cloned melanoma cell line (M 2669 CL13) was selected as the target and, when fixed with paraformaldehyde, showed binding as good as or better than that obtained with live cells for the three antigens. Fixed cells retained good binding properties stored at 4°C for over 6 mo. This assay has general applicability to other antigen-antibody systems for testing chemically modified monoclonal antibodies or fragments during the development of a radiopharmaceutical or as a routine quality control measure for clinical agents.",

author = "Beaumier, {P. L.} and D. Neuzil and Yang, {H. M.} and Noll, {E. A.} and R. Kishore and Eary, {J. F.} and Krohn, {K. A.} and Nelp, {W. B.} and Hellstr{\"o}m, {K. E.} and I. Hellstr{\"o}m",

year = "1986",

language = "English (US)",

volume = "27",

pages = "824--828",

journal = "Journal of Nuclear Medicine",

issn = "0161-5505",

publisher = "Society of Nuclear Medicine Inc.",

number = "6",

}

TY - JOUR

T1 - Immunoreactivity assay for labeled anti-melanoma monoclonal antibodies

AU - Beaumier, P. L.

AU - Neuzil, D.

AU - Yang, H. M.

AU - Noll, E. A.

AU - Kishore, R.

AU - Eary, J. F.

AU - Krohn, K. A.

AU - Nelp, W. B.

AU - Hellström, K. E.

AU - Hellström, I.

PY - 1986

Y1 - 1986

N2 - A convenient, rapid, and reproducible assay was developed to evaluate the immunoreactivity of radiolabeled monoclonal antibodies against three different human melanoma-associated antigens, p97, a proteoglycan and a GD3 ganglioside. A cloned melanoma cell line (M 2669 CL13) was selected as the target and, when fixed with paraformaldehyde, showed binding as good as or better than that obtained with live cells for the three antigens. Fixed cells retained good binding properties stored at 4°C for over 6 mo. This assay has general applicability to other antigen-antibody systems for testing chemically modified monoclonal antibodies or fragments during the development of a radiopharmaceutical or as a routine quality control measure for clinical agents.

AB - A convenient, rapid, and reproducible assay was developed to evaluate the immunoreactivity of radiolabeled monoclonal antibodies against three different human melanoma-associated antigens, p97, a proteoglycan and a GD3 ganglioside. A cloned melanoma cell line (M 2669 CL13) was selected as the target and, when fixed with paraformaldehyde, showed binding as good as or better than that obtained with live cells for the three antigens. Fixed cells retained good binding properties stored at 4°C for over 6 mo. This assay has general applicability to other antigen-antibody systems for testing chemically modified monoclonal antibodies or fragments during the development of a radiopharmaceutical or as a routine quality control measure for clinical agents.

UR - http://www.scopus.com/inward/record.url?scp=0022911183&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022911183&partnerID=8YFLogxK

M3 - Article

C2 - 3712094

AN - SCOPUS:0022911183

SN - 0161-5505

VL - 27

SP - 824

EP - 828

JO - Journal of Nuclear Medicine

JF - Journal of Nuclear Medicine

IS - 6

ER -

Immunoreactivity assay for labeled anti-melanoma monoclonal antibodies

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this