Impact of putamen inhibition by DREADDs on schedule-induced drinking in rhesus monkeys

Kathleen A. Grant, Natali N. Newman, Steven W. Gonzales, Verginia C. Cuzon Carlson

    Research output: Contribution to journalArticlepeer-review


    The putamen is a nucleus within the sensory-motor striatal network that is involved in automatic, habitual actions. Schedule-induced polydipsia (SIP) is highly automated behavior, reliably occurring under intermediate interval schedules of reinforcement. The effect of putamen inhibition in mediating SIP of water and ethanol (4% w/v) under a Fixed Time 5-min (FT-5 min) schedule for food delivery was tested in 12 rhesus monkeys (6 male, 6 female). Water and ethanol SIP sessions ended after set volumes were consumed. Baseline patterns of SIP intake differed between water and ethanol SIP in volume but not in pattern of drinking. Activation of the designer receptor exclusively activated by designer drug (DREADD: hM4Di) with deschloroclozapine (DCZ; 300 μg/kg, i.m.) administered 30 min prior to the onset of the SIP session, for four consecutive sessions. DCZ administration increased the postpellet drink volume and reduced the time to drink both water and ethanol. Although the effect of DCZ treatment was similar for increasing SIP with either water or ethanol, post-DCZ return to baseline SIP rates of differed, perhaps highlighting the effect of a state dependency with ethanol SIP. Overall, the study shows that targeting the putamen with the inhibitory DREADD produces a reversible, reproducible and reliable increase in adjunctive drinking.

    Original languageEnglish (US)
    Pages (from-to)493-504
    Number of pages12
    JournalJournal of the experimental analysis of behavior
    Issue number3
    StatePublished - May 2022


    • DREADDs
    • ethanol drinking
    • hM4Di
    • putamen
    • rhesus monkey
    • schedule induced polydipsia

    ASJC Scopus subject areas

    • Experimental and Cognitive Psychology
    • Behavioral Neuroscience


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