TY - CHAP
T1 - In Situ Multiplexing to Identify, Quantify, and Phenotype the HIV-1/SIV Reservoir Within Lymphoid Tissue
AU - Busman-Sahay, Kathleen
AU - Nekorchuk, Michael D.
AU - Starke, Carly Elizabeth
AU - Chan, Chi Ngai
AU - Estes, Jacob D.
N1 - Publisher Copyright:
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Modern combination antiretroviral therapy (ART) regimens provide abiding viral suppression for most individuals infected with human immunodeficiency virus (HIV). However, the persistence of viral reservoirs ensures that eradication of HIV-1 (i.e., cure) or sustained ART-free remission (i.e., functional cure) remains elusive, necessitating continual, strict ART adherence and contributing to HIV-1-related comorbidities. Eradication of these viral reservoirs, which persist primarily within lymphoid tissue, will require a deeper understanding of the cellular neighborhoods in which latent and active HIV-1-infected cells reside. By pairing highly sensitive in situ hybridization (ISH) with an exceptionally flexible immunofluorescence (IF) approach, we describe a simple, yet highly adaptable multiplex protocol for investigating the quantity, distribution, and characteristics of HIV-1 viral reservoirs.
AB - Modern combination antiretroviral therapy (ART) regimens provide abiding viral suppression for most individuals infected with human immunodeficiency virus (HIV). However, the persistence of viral reservoirs ensures that eradication of HIV-1 (i.e., cure) or sustained ART-free remission (i.e., functional cure) remains elusive, necessitating continual, strict ART adherence and contributing to HIV-1-related comorbidities. Eradication of these viral reservoirs, which persist primarily within lymphoid tissue, will require a deeper understanding of the cellular neighborhoods in which latent and active HIV-1-infected cells reside. By pairing highly sensitive in situ hybridization (ISH) with an exceptionally flexible immunofluorescence (IF) approach, we describe a simple, yet highly adaptable multiplex protocol for investigating the quantity, distribution, and characteristics of HIV-1 viral reservoirs.
KW - Fluorescent multiplex
KW - HIV
KW - Human immunodeficiency virus
KW - IF
KW - ISH
KW - Immunofluorescence
KW - In situ hybridization
KW - Lymphoid tissue
KW - Reservoir
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U2 - 10.1007/978-1-0716-1871-4_19
DO - 10.1007/978-1-0716-1871-4_19
M3 - Chapter
C2 - 34985671
AN - SCOPUS:85122780249
T3 - Methods in Molecular Biology
SP - 277
EP - 290
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -