TY - JOUR
T1 - In vitro antibody synthesis in 20 μl hanging drops
T2 - Initiation of secondary responses and a simple method of cloning hybridomas
AU - Bell, Eric B.
AU - Brown, McKay
AU - Rittenberg, Marvin B.
PY - 1983/8/26
Y1 - 1983/8/26
N2 - An in vitro culture system is described in which antibody synthesis is initiated by 105 hapten-primed spleen cells in 20 μl hanging drops. Plaque-forming cell (PFC) responses to thymus dependent and thymus independent antigens were obtained in drop cultures comparable to the micro Mishell-Dutton system. Antigens could be administered in soluble form or on antigen-pulsed peritoneal cells. The hanging drop method also provides a reliable yet simple procedure of cloning hybridoma cells while permitting direct visualization of the number of cells in each drop.
AB - An in vitro culture system is described in which antibody synthesis is initiated by 105 hapten-primed spleen cells in 20 μl hanging drops. Plaque-forming cell (PFC) responses to thymus dependent and thymus independent antigens were obtained in drop cultures comparable to the micro Mishell-Dutton system. Antigens could be administered in soluble form or on antigen-pulsed peritoneal cells. The hanging drop method also provides a reliable yet simple procedure of cloning hybridoma cells while permitting direct visualization of the number of cells in each drop.
KW - cell cloning
KW - hanging drop cultures
KW - hybridomas
KW - in vitro antibody synthesis
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U2 - 10.1016/0022-1759(83)90241-7
DO - 10.1016/0022-1759(83)90241-7
M3 - Article
C2 - 6350459
AN - SCOPUS:0020511156
SN - 0022-1759
VL - 62
SP - 137
EP - 145
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 2
ER -