In vitro replicative arrest in dnaAts mutants

Musetta A. Hanson; Robb E. Moses

doi:10.1016/0003-9861(78)90325-9

In vitro replicative arrest in dnaA_ts mutants

Musetta A. Hanson
, Robb E. Moses

Research output: Contribution to journal › Article › peer-review

Abstract

Toluene-treated Escherichia coli, conditionally defective in initiation of DNA replication, have been studied. Toluenized mutants of the dnaA_ts, class rapidly stop DNA synthesis at the restrictive temperature. This is in contrast to the slow arrest of replicative synthesis noted in vivo in these strains. The in vitro cessation of replicative DNA synthesis can be prevented by the presence of the detergent Triton X-100. Our results suggest a role in elongation of DNA by the dnaA gene product during replicative synthesis in vitro.

Original language	English (US)
Pages (from-to)	671-676
Number of pages	6
Journal	Archives of Biochemistry and Biophysics
Volume	190
Issue number	2
DOIs	https://doi.org/10.1016/0003-9861(78)90325-9
State	Published - Oct 1978
Externally published	Yes

Funding

This work wee supported by National Institutes of Health Grant GM-19122 and Robert A. Welch Foundation Grant No. Q-543. M.A.H. was supported by National Institutes of Health Grant GM-05421.

Funders	Funder number
Robert A. Welch Foundation	GM-05421
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	GM-19122

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

Access to Document

10.1016/0003-9861(78)90325-9

Cite this

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title = "In vitro replicative arrest in dnaAts mutants",

abstract = "Toluene-treated Escherichia coli, conditionally defective in initiation of DNA replication, have been studied. Toluenized mutants of the dnaAts, class rapidly stop DNA synthesis at the restrictive temperature. This is in contrast to the slow arrest of replicative synthesis noted in vivo in these strains. The in vitro cessation of replicative DNA synthesis can be prevented by the presence of the detergent Triton X-100. Our results suggest a role in elongation of DNA by the dnaA gene product during replicative synthesis in vitro.",

author = "Hanson, \{Musetta A.\} and Moses, \{Robb E.\}",

note = "Funding Information: This work wee supported by National Institutes of Health Grant GM-19122 and Robert A. Welch Foundation Grant No. Q-543. M.A.H. was supported by National Institutes of Health Grant GM-05421.",

year = "1978",

month = oct,

doi = "10.1016/0003-9861(78)90325-9",

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AU - Hanson, Musetta A.

AU - Moses, Robb E.

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N2 - Toluene-treated Escherichia coli, conditionally defective in initiation of DNA replication, have been studied. Toluenized mutants of the dnaAts, class rapidly stop DNA synthesis at the restrictive temperature. This is in contrast to the slow arrest of replicative synthesis noted in vivo in these strains. The in vitro cessation of replicative DNA synthesis can be prevented by the presence of the detergent Triton X-100. Our results suggest a role in elongation of DNA by the dnaA gene product during replicative synthesis in vitro.

AB - Toluene-treated Escherichia coli, conditionally defective in initiation of DNA replication, have been studied. Toluenized mutants of the dnaAts, class rapidly stop DNA synthesis at the restrictive temperature. This is in contrast to the slow arrest of replicative synthesis noted in vivo in these strains. The in vitro cessation of replicative DNA synthesis can be prevented by the presence of the detergent Triton X-100. Our results suggest a role in elongation of DNA by the dnaA gene product during replicative synthesis in vitro.

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