@inbook{9a9679f4b031430da33538ba0649c467,
title = "In vivo analysis of glial immune responses to axon degeneration in Drosophila melanogaster",
abstract = "Axon degeneration elicits a range of immune responses from local glial cells, including striking changes in glial gene expression, morphology, and phagocytic activity. Here, we describe a detailed set of protocols to assess discrete components of the glial reaction to axotomy in the adult nervous system of Drosophila melanogaster. These methods allow one to visualize and quantify transcriptional, morphological, and functional responses of glia to degenerating axons in a model system that is highly amenable to genetic manipulation.",
keywords = "Axotomy, Drosophila, Glia, Glial immunity, Neurodegeneration, Phagocytosis, Wallerian degeneration",
author = "Logan, {Mary A.} and Speese, {Sean D.}",
note = "Funding Information: We thank Maria Purice, Petra Richer, Derek Musashe, and Lilly Winfree for outstanding technical and intellectual contributions to this work. We thank Marc Freeman, Dirk Bohmann, Bloomington Drosophila Stock Center at Indiana University, and the Developmental Studies Hybridoma Bank at the University of Iowa for flies and antibodies. Funding: NIH R21 NS084112 (M.A.L. and S.D. S.), NIH R21 NS107771 (M.A.L.), NSF1557975 (S.D.S.), and the Ken and Ginger Harrison Term Professor Award (M.A.L.). Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-0716-0585-1_24",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "321--338",
booktitle = "Methods in Molecular Biology",
}