In vivo analysis of glial immune responses to axon degeneration in Drosophila melanogaster

Mary A. Logan; Sean D. Speese

doi:10.1007/978-1-0716-0585-1_24

In vivo analysis of glial immune responses to axon degeneration in Drosophila melanogaster

Mary A. Logan
, Sean D. Speese

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Scopus citations

Abstract

Axon degeneration elicits a range of immune responses from local glial cells, including striking changes in glial gene expression, morphology, and phagocytic activity. Here, we describe a detailed set of protocols to assess discrete components of the glial reaction to axotomy in the adult nervous system of Drosophila melanogaster. These methods allow one to visualize and quantify transcriptional, morphological, and functional responses of glia to degenerating axons in a model system that is highly amenable to genetic manipulation.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	321-338
Number of pages	18
DOIs	https://doi.org/10.1007/978-1-0716-0585-1_24
State	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	2143
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Funding

We thank Maria Purice, Petra Richer, Derek Musashe, and Lilly Winfree for outstanding technical and intellectual contributions to this work. We thank Marc Freeman, Dirk Bohmann, Bloomington Drosophila Stock Center at Indiana University, and the Developmental Studies Hybridoma Bank at the University of Iowa for flies and antibodies. Funding: NIH R21 NS084112 (M.A.L. and S.D. S.), NIH R21 NS107771 (M.A.L.), NSF1557975 (S.D.S.), and the Ken and Ginger Harrison Term Professor Award (M.A.L.).

Funders	Funder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	NSF1557975, R21 NS084112
National Institute of Neurological Disorders and Stroke	R21NS107771
Indiana University-Purdue University
University of Iowa Hospitals and Clinics, Iowa City

Keywords

Axotomy
Drosophila
Glia
Glial immunity
Neurodegeneration
Phagocytosis
Wallerian degeneration

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-0716-0585-1_24

Cite this

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abstract = "Axon degeneration elicits a range of immune responses from local glial cells, including striking changes in glial gene expression, morphology, and phagocytic activity. Here, we describe a detailed set of protocols to assess discrete components of the glial reaction to axotomy in the adult nervous system of Drosophila melanogaster. These methods allow one to visualize and quantify transcriptional, morphological, and functional responses of glia to degenerating axons in a model system that is highly amenable to genetic manipulation.",

keywords = "Axotomy, Drosophila, Glia, Glial immunity, Neurodegeneration, Phagocytosis, Wallerian degeneration",

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AU - Speese, Sean D.

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PY - 2020

Y1 - 2020

N2 - Axon degeneration elicits a range of immune responses from local glial cells, including striking changes in glial gene expression, morphology, and phagocytic activity. Here, we describe a detailed set of protocols to assess discrete components of the glial reaction to axotomy in the adult nervous system of Drosophila melanogaster. These methods allow one to visualize and quantify transcriptional, morphological, and functional responses of glia to degenerating axons in a model system that is highly amenable to genetic manipulation.

AB - Axon degeneration elicits a range of immune responses from local glial cells, including striking changes in glial gene expression, morphology, and phagocytic activity. Here, we describe a detailed set of protocols to assess discrete components of the glial reaction to axotomy in the adult nervous system of Drosophila melanogaster. These methods allow one to visualize and quantify transcriptional, morphological, and functional responses of glia to degenerating axons in a model system that is highly amenable to genetic manipulation.

KW - Axotomy

KW - Drosophila

KW - Glia

KW - Glial immunity

KW - Neurodegeneration

KW - Phagocytosis

KW - Wallerian degeneration

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BT - Methods in Molecular Biology

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ER -

In vivo analysis of glial immune responses to axon degeneration in Drosophila melanogaster

Abstract

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Keywords

ASJC Scopus subject areas

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