In vivo profiling and visualization of cellular protein-lipid interactions using bifunctional fatty acids

Per Haberkant; Reinout Raijmakers; Marjolein Wildwater; Timo Sachsenheimer; Britta Brügger; Kenji Maeda; Martin Houweling; Anne Claude Gavin; Carsten Schultz; Gerrit Van Meer; Albert J.R. Heck; Joost C.M. Holthuis

doi:10.1002/anie.201210178

In vivo profiling and visualization of cellular protein-lipid interactions using bifunctional fatty acids

Per Haberkant, Reinout Raijmakers, Marjolein Wildwater, Timo Sachsenheimer, Britta Brügger, Kenji Maeda, Martin Houweling, Anne Claude Gavin, Carsten Schultz, Gerrit Van Meer, Albert J.R. Heck, Joost C.M. Holthuis

Research output: Contribution to journal › Article › peer-review

98 Scopus citations

Abstract

Bifunctional lipid technology: Cells convert externally added photoactivatable and "clickable" fatty acids into a variety of bifunctional phospholipids that can be covalently linked to their protein-binding partners by irradiation with UV light. Derivatization of the clickable group with a reporter molecule makes it possible to identify and image the lipid-bound proteins in situ (see scheme).

Original language	English (US)
Pages (from-to)	4033-4038
Number of pages	6
Journal	Angewandte Chemie - International Edition
Volume	52
Issue number	14
DOIs	https://doi.org/10.1002/anie.201210178
State	Published - Apr 2 2013
Externally published	Yes

Keywords

cell imaging
click chemistry
lipids
photoaffinity labeling
proteins

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.201210178

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Haberkant, P., Raijmakers, R., Wildwater, M., Sachsenheimer, T., Brügger, B., Maeda, K., Houweling, M., Gavin, A. C., Schultz, C., Van Meer, G., Heck, A. J. R., & Holthuis, J. C. M. (2013). In vivo profiling and visualization of cellular protein-lipid interactions using bifunctional fatty acids. Angewandte Chemie - International Edition, 52(14), 4033-4038. https://doi.org/10.1002/anie.201210178

Haberkant, P, Raijmakers, R, Wildwater, M, Sachsenheimer, T, Brügger, B, Maeda, K, Houweling, M, Gavin, AC, Schultz, C, Van Meer, G, Heck, AJR & Holthuis, JCM 2013, 'In vivo profiling and visualization of cellular protein-lipid interactions using bifunctional fatty acids', Angewandte Chemie - International Edition, vol. 52, no. 14, pp. 4033-4038. https://doi.org/10.1002/anie.201210178

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AU - Brügger, Britta

AU - Maeda, Kenji

AU - Houweling, Martin

AU - Gavin, Anne Claude

AU - Schultz, Carsten

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AU - Heck, Albert J.R.

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In vivo profiling and visualization of cellular protein-lipid interactions using bifunctional fatty acids

Abstract

Keywords

ASJC Scopus subject areas

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