TY - JOUR
T1 - Inactive X chromosome DNA does not function in DNA-mediated cell transformation for the hypoxanthine phosphoribosyltransferase gene.
AU - Liskay, R. M.
AU - Evans, R. J.
PY - 1980/8
Y1 - 1980/8
N2 - The molecular nature of the X chromosome inactivation process has been investigated by utilizing the techniques of DNA-mediated cell transformation of the X-linked hypoxanthine phosphoribosyltransferase (HPRT) locus. The findings indicate that purified DNA from the inactive X chromosome of a near-euploid mouse cell line is not functional in transformation for HPRT, but the DNA from its "homologous" active X readily elicits transformation for HPRT in the same hamster cell recipient. These findings suggest that there is a difference between the DNA, per se, of the active and inactive X at (or near) the HPRT locus and that this difference could account, at least in part, for its inactivation.
AB - The molecular nature of the X chromosome inactivation process has been investigated by utilizing the techniques of DNA-mediated cell transformation of the X-linked hypoxanthine phosphoribosyltransferase (HPRT) locus. The findings indicate that purified DNA from the inactive X chromosome of a near-euploid mouse cell line is not functional in transformation for HPRT, but the DNA from its "homologous" active X readily elicits transformation for HPRT in the same hamster cell recipient. These findings suggest that there is a difference between the DNA, per se, of the active and inactive X at (or near) the HPRT locus and that this difference could account, at least in part, for its inactivation.
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U2 - 10.1073/pnas.77.8.4895
DO - 10.1073/pnas.77.8.4895
M3 - Article
C2 - 6933537
AN - SCOPUS:0019051757
SN - 0027-8424
VL - 77
SP - 4895
EP - 4898
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 8
ER -