Incorporation of aerobic oxygen into the hydroxyglycyl intermediate during formation of C-terminal peptide amides by peptidylglycine α-amidating monooxygenase (PAM)

T. Mark Zabriskie; Hengmiao Cheng; John C. Vederas

doi:10.1039/C39910000571

Incorporation of aerobic oxygen into the hydroxyglycyl intermediate during formation of C-terminal peptide amides by peptidylglycine α-amidating monooxygenase (PAM)

T. Mark Zabriskie, Hengmiao Cheng, John C. Vederas

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Incubation of D-phenylalanyl-L-phenylalanylglycine with peptidylglycine α-amidating monooxygenase (PAM) in the presence of ¹⁸O ₂ generates the corresponding hydroxyglycyl peptide whose FAB mass spectrum shows high incorporation of ¹⁸O into the hydroxy group; this suggests that the mechanism may involve direct hydroxylation of carbon.

Original language	English (US)
Pages (from-to)	571-572
Number of pages	2
Journal	Journal of the Chemical Society, Chemical Communications
Issue number	8
DOIs	https://doi.org/10.1039/C39910000571
State	Published - 1991
Externally published	Yes

ASJC Scopus subject areas

Molecular Medicine

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10.1039/C39910000571

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Incorporation of aerobic oxygen into the hydroxyglycyl intermediate during formation of C-terminal peptide amides by peptidylglycine α-amidating monooxygenase (PAM). / Zabriskie, T. Mark; Cheng, Hengmiao; Vederas, John C.
In: Journal of the Chemical Society, Chemical Communications, No. 8, 1991, p. 571-572.

Research output: Contribution to journal › Article › peer-review

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title = "Incorporation of aerobic oxygen into the hydroxyglycyl intermediate during formation of C-terminal peptide amides by peptidylglycine α-amidating monooxygenase (PAM)",

abstract = "Incubation of D-phenylalanyl-L-phenylalanylglycine with peptidylglycine α-amidating monooxygenase (PAM) in the presence of 18O 2 generates the corresponding hydroxyglycyl peptide whose FAB mass spectrum shows high incorporation of 18O into the hydroxy group; this suggests that the mechanism may involve direct hydroxylation of carbon.",

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AU - Vederas, John C.

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Incorporation of aerobic oxygen into the hydroxyglycyl intermediate during formation of C-terminal peptide amides by peptidylglycine α-amidating monooxygenase (PAM)

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