TY - JOUR
T1 - Incorporation of Apigenin and tt-Farnesol into dental composites to modulate the Streptococcus mutans virulence
AU - André, Carolina Bosso
AU - Rosalen, Pedro Luiz
AU - Giannini, Marcelo
AU - Bueno-Silva, Bruno
AU - Pfeifer, Carmem Silvia
AU - Ferracane, Jack Liborio
N1 - Publisher Copyright:
© 2020 The Academy of Dental Materials
PY - 2021/4
Y1 - 2021/4
N2 - Objectives: The aim of this in vitro study was to incorporate two anti-caries agents, Apigenin and tt-Farnesol, to resin composite and resin cement to reduce the virulence of Streptococcus mutans around dental restorations. Methods: Apigenin (Api, 5 mM) and tt-Farnesol (Far, 5 mM) were added alone, together, and combined with fluoride (F). Biofilm of S. mutans was grown on composite discs, and the dry-weight, bacterial viability, and the polysaccharides (alkali-soluble, intracellular and water-soluble) were quantified. CLSM images of the S. mutans biofilm were obtained after three years of water-storage. The effect of the additions on the physicochemical properties and the composite colorimetric parameters were also analyzed. Results: The additions did not affect bacterial viability. Api alone and combined with Far or combined with Far and F decreased the bacterial dry-weight, alkali-soluble and intracellular polysaccharides. After three years, the composites containing the additions presented a greater EPS matrix on the top of biofilm. Statistical difference was obtained for the degree of conversion; however, the maximum polymerization rate and curing kinetics were unaffected by the additions. No difference was observed for the water-soluble polysaccharides, flexural strength, and elastic modulus. Api increased the yellowness of the composites. Significance: Api, alone and combined, reduced the expression of virulence of S. mutans without jeopardizing the physicochemical properties of the composites.
AB - Objectives: The aim of this in vitro study was to incorporate two anti-caries agents, Apigenin and tt-Farnesol, to resin composite and resin cement to reduce the virulence of Streptococcus mutans around dental restorations. Methods: Apigenin (Api, 5 mM) and tt-Farnesol (Far, 5 mM) were added alone, together, and combined with fluoride (F). Biofilm of S. mutans was grown on composite discs, and the dry-weight, bacterial viability, and the polysaccharides (alkali-soluble, intracellular and water-soluble) were quantified. CLSM images of the S. mutans biofilm were obtained after three years of water-storage. The effect of the additions on the physicochemical properties and the composite colorimetric parameters were also analyzed. Results: The additions did not affect bacterial viability. Api alone and combined with Far or combined with Far and F decreased the bacterial dry-weight, alkali-soluble and intracellular polysaccharides. After three years, the composites containing the additions presented a greater EPS matrix on the top of biofilm. Statistical difference was obtained for the degree of conversion; however, the maximum polymerization rate and curing kinetics were unaffected by the additions. No difference was observed for the water-soluble polysaccharides, flexural strength, and elastic modulus. Api increased the yellowness of the composites. Significance: Api, alone and combined, reduced the expression of virulence of S. mutans without jeopardizing the physicochemical properties of the composites.
KW - Apigenin
KW - Dental caries
KW - Resin cement
KW - Resin composite
KW - Streptococcus mutans
KW - tt-Farnesol
UR - http://www.scopus.com/inward/record.url?scp=85099146801&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85099146801&partnerID=8YFLogxK
U2 - 10.1016/j.dental.2020.12.005
DO - 10.1016/j.dental.2020.12.005
M3 - Article
C2 - 33422299
AN - SCOPUS:85099146801
SN - 0109-5641
VL - 37
SP - e201-e212
JO - Dental Materials
JF - Dental Materials
IS - 4
ER -