Increased platelet Fc receptor expression as a potential contributing cause of platelet hypersensitivity to collagen in diabetes mellitus

David C. Calverley; Michele R. Hacker; Kimberly A. Loda; Elizabeth Brass; Thomas A. Buchanan; Denice D. Tsao-Wei; Susan Groshen

doi:10.1046/j.1365-2141.2003.04233.x

Increased platelet Fc receptor expression as a potential contributing cause of platelet hypersensitivity to collagen in diabetes mellitus

David C. Calverley, Michele R. Hacker, Kimberly A. Loda, Elizabeth Brass, Thomas A. Buchanan, Denice D. Tsao-Wei, Susan Groshen

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Recent clinical and laboratory observations support a potential role for the platelet FcγRIIA receptor (FcR) in collagen-mediated platelet activation associated with arterial thrombosis. Stable age- and sex-independent variation in receptor expression exists. Flow cytometry showed that 100 diabetes patients had increased mean platelet FcR expression levels compared with 201 non-diabetes patients (P < 0.001). Aggregation studies following FcR cross-linking supported a receptor role in increased activation of diabetes platelets after collagen stimulation (P = 0.018). Immunoprecipitation of collagen-stimulated and FcR-crosslinked platelets demonstrated enhanced levels of tyrosine-phosphorylated Syk in diabetic platelets. Increased platelet FcR-mediated sensitivity to collagen may contribute to cardiovascular morbidity and mortality in diabetes.

Original language	English (US)
Pages (from-to)	139-142
Number of pages	4
Journal	British Journal of Haematology
Volume	121
Issue number	1
DOIs	https://doi.org/10.1046/j.1365-2141.2003.04233.x
State	Published - Apr 2003
Externally published	Yes

Keywords

Blood platelets
Cardiovascular disease
Collagen
Diabetes mellitus
Fc
Receptors

ASJC Scopus subject areas

Hematology

Access to Document

10.1046/j.1365-2141.2003.04233.x

Cite this

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title = "Increased platelet Fc receptor expression as a potential contributing cause of platelet hypersensitivity to collagen in diabetes mellitus",

abstract = "Recent clinical and laboratory observations support a potential role for the platelet FcγRIIA receptor (FcR) in collagen-mediated platelet activation associated with arterial thrombosis. Stable age- and sex-independent variation in receptor expression exists. Flow cytometry showed that 100 diabetes patients had increased mean platelet FcR expression levels compared with 201 non-diabetes patients (P < 0.001). Aggregation studies following FcR cross-linking supported a receptor role in increased activation of diabetes platelets after collagen stimulation (P = 0.018). Immunoprecipitation of collagen-stimulated and FcR-crosslinked platelets demonstrated enhanced levels of tyrosine-phosphorylated Syk in diabetic platelets. Increased platelet FcR-mediated sensitivity to collagen may contribute to cardiovascular morbidity and mortality in diabetes.",

keywords = "Blood platelets, Cardiovascular disease, Collagen, Diabetes mellitus, Fc, Receptors",

author = "Calverley, {David C.} and Hacker, {Michele R.} and Loda, {Kimberly A.} and Elizabeth Brass and Buchanan, {Thomas A.} and Tsao-Wei, {Denice D.} and Susan Groshen",

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doi = "10.1046/j.1365-2141.2003.04233.x",

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AU - Calverley, David C.

AU - Hacker, Michele R.

AU - Loda, Kimberly A.

AU - Brass, Elizabeth

AU - Buchanan, Thomas A.

AU - Tsao-Wei, Denice D.

AU - Groshen, Susan

PY - 2003/4

Y1 - 2003/4

N2 - Recent clinical and laboratory observations support a potential role for the platelet FcγRIIA receptor (FcR) in collagen-mediated platelet activation associated with arterial thrombosis. Stable age- and sex-independent variation in receptor expression exists. Flow cytometry showed that 100 diabetes patients had increased mean platelet FcR expression levels compared with 201 non-diabetes patients (P < 0.001). Aggregation studies following FcR cross-linking supported a receptor role in increased activation of diabetes platelets after collagen stimulation (P = 0.018). Immunoprecipitation of collagen-stimulated and FcR-crosslinked platelets demonstrated enhanced levels of tyrosine-phosphorylated Syk in diabetic platelets. Increased platelet FcR-mediated sensitivity to collagen may contribute to cardiovascular morbidity and mortality in diabetes.

AB - Recent clinical and laboratory observations support a potential role for the platelet FcγRIIA receptor (FcR) in collagen-mediated platelet activation associated with arterial thrombosis. Stable age- and sex-independent variation in receptor expression exists. Flow cytometry showed that 100 diabetes patients had increased mean platelet FcR expression levels compared with 201 non-diabetes patients (P < 0.001). Aggregation studies following FcR cross-linking supported a receptor role in increased activation of diabetes platelets after collagen stimulation (P = 0.018). Immunoprecipitation of collagen-stimulated and FcR-crosslinked platelets demonstrated enhanced levels of tyrosine-phosphorylated Syk in diabetic platelets. Increased platelet FcR-mediated sensitivity to collagen may contribute to cardiovascular morbidity and mortality in diabetes.

KW - Blood platelets

KW - Cardiovascular disease

KW - Collagen

KW - Diabetes mellitus

KW - Fc

KW - Receptors

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Increased platelet Fc receptor expression as a potential contributing cause of platelet hypersensitivity to collagen in diabetes mellitus

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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