Influence of pentobarbital on synaptic plasma membrane protein synthesis

Robert J. Hitzemann; Horace H. Loh

doi:10.1016/0024-3205(77)90125-4

Influence of pentobarbital on synaptic plasma membrane protein synthesis

Robert J. Hitzemann, Horace H. Loh

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The effects of acute and chronic pentobarbital treatment on the incorporation of ³H-lysine into rat brain synaptic plasma membranes (SPM) were examined by means of SDS polyacrylamide gel electrophoresis. The data suggest the major effects of both drug treatments are found in a unique population of SPM derived from a light population of nerve ending particles (NEP). These light NEP preferentially accumulate labelled GABA, suggesting this fraction is enriched in GABA containing nerve terminals.

Original language	English (US)
Pages (from-to)	35-41
Number of pages	7
Journal	Life Sciences
Volume	20
Issue number	1
DOIs	https://doi.org/10.1016/0024-3205(77)90125-4
State	Published - Jan 1 1977
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(77)90125-4

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title = "Influence of pentobarbital on synaptic plasma membrane protein synthesis",

abstract = "The effects of acute and chronic pentobarbital treatment on the incorporation of 3H-lysine into rat brain synaptic plasma membranes (SPM) were examined by means of SDS polyacrylamide gel electrophoresis. The data suggest the major effects of both drug treatments are found in a unique population of SPM derived from a light population of nerve ending particles (NEP). These light NEP preferentially accumulate labelled GABA, suggesting this fraction is enriched in GABA containing nerve terminals.",

author = "Hitzemann, {Robert J.} and Loh, {Horace H.}",

note = "Funding Information: Male Sprague-Dawley rats (Simonsen Laboratories, Gilroy, CA .) weighing 180-200 g were implanted with a needle guide above the lateral ventricle. Four to 7 days later, the Snimals were implanted with two placebo or two 200 mg ;This study was supported in part by USPHS Grant DA-OOS64. ent of a NINE Research Scientist Career Development Award,",

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AU - Loh, Horace H.

N1 - Funding Information: Male Sprague-Dawley rats (Simonsen Laboratories, Gilroy, CA .) weighing 180-200 g were implanted with a needle guide above the lateral ventricle. Four to 7 days later, the Snimals were implanted with two placebo or two 200 mg ;This study was supported in part by USPHS Grant DA-OOS64. ent of a NINE Research Scientist Career Development Award,

PY - 1977/1/1

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N2 - The effects of acute and chronic pentobarbital treatment on the incorporation of 3H-lysine into rat brain synaptic plasma membranes (SPM) were examined by means of SDS polyacrylamide gel electrophoresis. The data suggest the major effects of both drug treatments are found in a unique population of SPM derived from a light population of nerve ending particles (NEP). These light NEP preferentially accumulate labelled GABA, suggesting this fraction is enriched in GABA containing nerve terminals.

AB - The effects of acute and chronic pentobarbital treatment on the incorporation of 3H-lysine into rat brain synaptic plasma membranes (SPM) were examined by means of SDS polyacrylamide gel electrophoresis. The data suggest the major effects of both drug treatments are found in a unique population of SPM derived from a light population of nerve ending particles (NEP). These light NEP preferentially accumulate labelled GABA, suggesting this fraction is enriched in GABA containing nerve terminals.

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Influence of pentobarbital on synaptic plasma membrane protein synthesis

Abstract

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