Insulin reduces norepinephrine transporter mRNA in vivo in rat locus coeruleus

Dianne P. Figlewicz; Patricia Szot; Paige A. Israel; Christopher Payne; Daniel M. Dorsa

doi:10.1016/0006-8993(93)90258-O

Insulin reduces norepinephrine transporter mRNA in vivo in rat locus coeruleus

Dianne P. Figlewicz
, Patricia Szot
, Paige A. Israel
, Christopher Payne
, Daniel M. Dorsa

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

Acute and chronic in vitro insulin treatment can inhibit the uptake of norepinephrine (NE) by adult rat brain synaptosomes and slices, fetal neuronal cultures, and PC12 cells. In the present study we tested whether chronic in vivo insulin treatment could alter the biosynthetic capacity of rat locus coeruleus neurons for the NE transporter protein (NET). Chronic third ventricular insulin treatment resulted in a suppression of NET mRNA to about one third of the level of vehicle-treated controls. Our findings suggests that insulin may play a regulatory role in the synthesis of this transporter, thereby modulating activity in CNS noradrenergic pathways.

Original language	English (US)
Pages (from-to)	161-164
Number of pages	4
Journal	Brain Research
Volume	602
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(93)90258-O
State	Published - Jan 29 1993
Externally published	Yes

Funding

The authors gratefully acknowledge the superb technical support of Sylvia White and we thank Dr. James N. Bicknell for his helpful support. Porcine insulin was the generous gift of Eli Lilly Corp., Indianapolis, IN. Funding for this research was provided by NIH grants DK-40963 (D.P.F.) and NS-20311 (D.M.D.); and the Research Service of the Department of Veterans Affairs.

Funders	Funder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	DK-40963
National Institute of Neurological Disorders and Stroke	R01NS020311
U.S. Department of Veterans Affairs

Keywords

Catecholamine
Insulin
Locus ceruleus
Norepinephrine transporter

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(93)90258-O

Cite this

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title = "Insulin reduces norepinephrine transporter mRNA in vivo in rat locus coeruleus",

abstract = "Acute and chronic in vitro insulin treatment can inhibit the uptake of norepinephrine (NE) by adult rat brain synaptosomes and slices, fetal neuronal cultures, and PC12 cells. In the present study we tested whether chronic in vivo insulin treatment could alter the biosynthetic capacity of rat locus coeruleus neurons for the NE transporter protein (NET). Chronic third ventricular insulin treatment resulted in a suppression of NET mRNA to about one third of the level of vehicle-treated controls. Our findings suggests that insulin may play a regulatory role in the synthesis of this transporter, thereby modulating activity in CNS noradrenergic pathways.",

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note = "Funding Information: The authors gratefully acknowledge the superb technical support of Sylvia White and we thank Dr. James N. Bicknell for his helpful support. Porcine insulin was the generous gift of Eli Lilly Corp., Indianapolis, IN. Funding for this research was provided by NIH grants DK-40963 (D.P.F.) and NS-20311 (D.M.D.); and the Research Service of the Department of Veterans Affairs.",

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AU - Figlewicz, Dianne P.

AU - Szot, Patricia

AU - Israel, Paige A.

AU - Payne, Christopher

AU - Dorsa, Daniel M.

N1 - Funding Information: The authors gratefully acknowledge the superb technical support of Sylvia White and we thank Dr. James N. Bicknell for his helpful support. Porcine insulin was the generous gift of Eli Lilly Corp., Indianapolis, IN. Funding for this research was provided by NIH grants DK-40963 (D.P.F.) and NS-20311 (D.M.D.); and the Research Service of the Department of Veterans Affairs.

PY - 1993/1/29

Y1 - 1993/1/29

N2 - Acute and chronic in vitro insulin treatment can inhibit the uptake of norepinephrine (NE) by adult rat brain synaptosomes and slices, fetal neuronal cultures, and PC12 cells. In the present study we tested whether chronic in vivo insulin treatment could alter the biosynthetic capacity of rat locus coeruleus neurons for the NE transporter protein (NET). Chronic third ventricular insulin treatment resulted in a suppression of NET mRNA to about one third of the level of vehicle-treated controls. Our findings suggests that insulin may play a regulatory role in the synthesis of this transporter, thereby modulating activity in CNS noradrenergic pathways.

AB - Acute and chronic in vitro insulin treatment can inhibit the uptake of norepinephrine (NE) by adult rat brain synaptosomes and slices, fetal neuronal cultures, and PC12 cells. In the present study we tested whether chronic in vivo insulin treatment could alter the biosynthetic capacity of rat locus coeruleus neurons for the NE transporter protein (NET). Chronic third ventricular insulin treatment resulted in a suppression of NET mRNA to about one third of the level of vehicle-treated controls. Our findings suggests that insulin may play a regulatory role in the synthesis of this transporter, thereby modulating activity in CNS noradrenergic pathways.

KW - Catecholamine

KW - Insulin

KW - Locus ceruleus

KW - Norepinephrine transporter

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JO - Brain Research

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Insulin reduces norepinephrine transporter mRNA in vivo in rat locus coeruleus

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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