Integrative analysis on histopathological image for identifying cellular heterogeneity

Young Hwan Chang, Guillaume Thibault, Brett Johnson, Adam Margolin, Joe W. Gray

Research output: Chapter in Book/Report/Conference proceedingConference contribution


This study has brought together image processing, clustering and spatial pattern analysis to quantitatively analyze hematoxylin and eosin-stained (H&E) tissue sections. A mixture of tumor and normal cells (intratumoral heterogeneity) as well as complex tissue architectures of most samples complicate the interpretation of their cytological profiles. To address these challenges, we develop a simple but effective methodology for quantitative analysis for H&E section. We adopt comparative analyses of spatial point patterns to characterize spatial distribution of different nuclei types and complement cellular characteristics analysis. We demonstrate that tumor and normal cell regions exhibit significant differences of lymphocytes spatial distribution or lymphocyte infiltration pattern.

Original languageEnglish (US)
Title of host publicationMedical Imaging 2017
Subtitle of host publicationDigital Pathology
EditorsMetin N. Gurcan, John E. Tomaszewski
ISBN (Electronic)9781510607255
StatePublished - 2017
EventMedical Imaging 2017: Digital Pathology - Orlando, United States
Duration: Feb 12 2017Feb 13 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherMedical Imaging 2017: Digital Pathology
Country/TerritoryUnited States


  • H&E stained image
  • Heterogeneity
  • Spatial pattern analysis

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


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