Abstract
Background: Th2 cytokines play pivotal roles in allergic inflammation, including eosinophilia, and their actions are antagonized by Th1 cytokines, conferring them therapeutic potential. Methods: In this study, we examined the ability of a number of cytokines to suppress the activation of eosinophils that function as effector cells for allergic airway diseases. Results: Interleukin (IL)-5, IL-6, and tumor necrosis factor (TNF) induced an eosinophil shape change, whereas interferon (IFN)-γ significantly inhibited the shape change. Other cytokines, including IL-1β, IL-4, IL-10 and IL-13, had little or only slightly enhancing or reducing effects on the shape change. We further analyzed the IFN-γ effect, showing that pretreatment with IFN-γ strongly suppressed IL-5-induced eosinophil shape change, and cycloheximide (CHX) abrogated the suppression by IFN-γ, suggesting that new protein synthesis is required for the inhibitory effect by this cytokine. In agreement with these results, IFN-γ blocked the eosinophil migration and ERK phophorylation induced by IL-5, and the addition of CHX restored eosinophil chemotaxis. Conclusions: Collectively, IFN-γ may attenuate eosinophilic inflammation by directly negating eosinophil mobilization.
Original language | English (US) |
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Pages (from-to) | 295-302 |
Number of pages | 8 |
Journal | International Archives of Allergy and Immunology |
Volume | 136 |
Issue number | 3 |
DOIs | |
State | Published - Mar 2005 |
Externally published | Yes |
Keywords
- Asthma
- Eosinophils
- Interferon-γ
- Interleukin-5
- Interleukin-6
- Tumor necrosis factor
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology