TY - JOUR
T1 - Intestinal growth-promoting properties of glucagon-like peptide-2 in mice
AU - Tsai, Chun Hui
AU - Hill, Mary
AU - Asa, Sylvia L.
AU - Brubaker, Patricia L.
AU - Drucker, Daniel J.
PY - 1997/7
Y1 - 1997/7
N2 - Glucagon-like peptide-2 (GLP-2) has been shown to promote intestinal epithelial proliferation. We studied crypt cell proliferation, enterocyte cell death, and feeding behavior in GLP-2-treated mice GLP-2 had no effect on food consumption [7.7 ± 0.3 vs. 8.0 ± 0.4 g/day, saline (control) vs. GLP- 2-treated mice, P = not significant]; however, GLP-2 increased the crypt cell proliferation rate (46.0 ± 1 vs. 57 ± 5%, control vs. GLP-2, P < 0.01) and decreased the enterocyte apoptotic rate (5.9 ± 0.7 vs. 2.8 ± 0.2% apoptotic cells, control vs. GLP-2, P < 0.05) in small bowel (SB) epithelium. GLP-2 induced a significant increase in SB weight (1.3- to 1.75-fold increase over control, P < 0.05 to P < 0.001) in mice 1-24 mo of age. Increased SB weight was maintained after daily administration of GLP-2 to mice for 12 wk, and cessation of GLP-2 administration in older mice led to regression of (increased) SB weight and mucosal height. These observations suggest that GLP-2 regulates both cell proliferation and apoptosis and promotes intestinal growth after both short- and long-term administration in vivo.
AB - Glucagon-like peptide-2 (GLP-2) has been shown to promote intestinal epithelial proliferation. We studied crypt cell proliferation, enterocyte cell death, and feeding behavior in GLP-2-treated mice GLP-2 had no effect on food consumption [7.7 ± 0.3 vs. 8.0 ± 0.4 g/day, saline (control) vs. GLP- 2-treated mice, P = not significant]; however, GLP-2 increased the crypt cell proliferation rate (46.0 ± 1 vs. 57 ± 5%, control vs. GLP-2, P < 0.01) and decreased the enterocyte apoptotic rate (5.9 ± 0.7 vs. 2.8 ± 0.2% apoptotic cells, control vs. GLP-2, P < 0.05) in small bowel (SB) epithelium. GLP-2 induced a significant increase in SB weight (1.3- to 1.75-fold increase over control, P < 0.05 to P < 0.001) in mice 1-24 mo of age. Increased SB weight was maintained after daily administration of GLP-2 to mice for 12 wk, and cessation of GLP-2 administration in older mice led to regression of (increased) SB weight and mucosal height. These observations suggest that GLP-2 regulates both cell proliferation and apoptosis and promotes intestinal growth after both short- and long-term administration in vivo.
KW - Apoptosis
KW - Bowel
KW - Feeding
KW - Growth factor
UR - http://www.scopus.com/inward/record.url?scp=0030759264&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030759264&partnerID=8YFLogxK
U2 - 10.1152/ajpendo.1997.273.1.e77
DO - 10.1152/ajpendo.1997.273.1.e77
M3 - Article
C2 - 9252482
AN - SCOPUS:0030759264
SN - 0193-1849
VL - 273
SP - E77-E84
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
IS - 1 36-1
ER -