Intracellular imaging of HCV RNA and cellular lipids by using simultaneous two-photon fluorescence and coherent anti-stokes Raman scattering microscopies

Xiaolin Nan; Angela M. Tonary; Albert Stolow; X. Sunney Xie; John Paul Pezacki

doi:10.1002/cbic.200600330

Intracellular imaging of HCV RNA and cellular lipids by using simultaneous two-photon fluorescence and coherent anti-stokes Raman scattering microscopies

Xiaolin Nan, Angela M. Tonary, Albert Stolow, X. Sunney Xie, John Paul Pezacki

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

Hepatitis C virus (HCV) infection is associated with changes in host-cell lipid metabolism. Here we describe a new approach for detecting HCV RNA using two-photon fluorescence (TPF), and HCV-associated changes in cellular lipids using coherent anti-Stokes Raman scattering (CARS) microscopy. By combining the two types of microscopy with a common laser source, we visualized both phenomena simultaneously and profiled cellular lipids and subcellular localization of RNA in real time.

Original language	English (US)
Pages (from-to)	1895-1897
Number of pages	3
Journal	ChemBioChem
Volume	7
Issue number	12
DOIs	https://doi.org/10.1002/cbic.200600330
State	Published - Dec 2006
Externally published	Yes

Keywords

CARS microscopy
Hepatitis C virus
Host-viral interactions
RNA
Two-photon fluorescence

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.200600330

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title = "Intracellular imaging of HCV RNA and cellular lipids by using simultaneous two-photon fluorescence and coherent anti-stokes Raman scattering microscopies",

abstract = "Hepatitis C virus (HCV) infection is associated with changes in host-cell lipid metabolism. Here we describe a new approach for detecting HCV RNA using two-photon fluorescence (TPF), and HCV-associated changes in cellular lipids using coherent anti-Stokes Raman scattering (CARS) microscopy. By combining the two types of microscopy with a common laser source, we visualized both phenomena simultaneously and profiled cellular lipids and subcellular localization of RNA in real time.",

keywords = "CARS microscopy, Hepatitis C virus, Host-viral interactions, RNA, Two-photon fluorescence",

author = "Xiaolin Nan and Tonary, {Angela M.} and Albert Stolow and Xie, {X. Sunney} and Pezacki, {John Paul}",

year = "2006",

month = dec,

doi = "10.1002/cbic.200600330",

language = "English (US)",

volume = "7",

pages = "1895--1897",

journal = "ChemBioChem",

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T1 - Intracellular imaging of HCV RNA and cellular lipids by using simultaneous two-photon fluorescence and coherent anti-stokes Raman scattering microscopies

AU - Nan, Xiaolin

AU - Tonary, Angela M.

AU - Stolow, Albert

AU - Xie, X. Sunney

AU - Pezacki, John Paul

PY - 2006/12

Y1 - 2006/12

N2 - Hepatitis C virus (HCV) infection is associated with changes in host-cell lipid metabolism. Here we describe a new approach for detecting HCV RNA using two-photon fluorescence (TPF), and HCV-associated changes in cellular lipids using coherent anti-Stokes Raman scattering (CARS) microscopy. By combining the two types of microscopy with a common laser source, we visualized both phenomena simultaneously and profiled cellular lipids and subcellular localization of RNA in real time.

AB - Hepatitis C virus (HCV) infection is associated with changes in host-cell lipid metabolism. Here we describe a new approach for detecting HCV RNA using two-photon fluorescence (TPF), and HCV-associated changes in cellular lipids using coherent anti-Stokes Raman scattering (CARS) microscopy. By combining the two types of microscopy with a common laser source, we visualized both phenomena simultaneously and profiled cellular lipids and subcellular localization of RNA in real time.

KW - CARS microscopy

KW - Hepatitis C virus

KW - Host-viral interactions

KW - RNA

KW - Two-photon fluorescence

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EP - 1897

JO - ChemBioChem

JF - ChemBioChem

IS - 12

ER -

Intracellular imaging of HCV RNA and cellular lipids by using simultaneous two-photon fluorescence and coherent anti-stokes Raman scattering microscopies

Abstract

Keywords

ASJC Scopus subject areas

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