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Intracellular imaging of HCV RNA and cellular lipids by using simultaneous two-photon fluorescence and coherent anti-stokes Raman scattering microscopies

  • Xiaolin Nan
  • , Angela M. Tonary
  • , Albert Stolow
  • , X. Sunney Xie
  • , John Paul Pezacki

Research output: Contribution to journalArticlepeer-review

Abstract

Hepatitis C virus (HCV) infection is associated with changes in host-cell lipid metabolism. Here we describe a new approach for detecting HCV RNA using two-photon fluorescence (TPF), and HCV-associated changes in cellular lipids using coherent anti-Stokes Raman scattering (CARS) microscopy. By combining the two types of microscopy with a common laser source, we visualized both phenomena simultaneously and profiled cellular lipids and subcellular localization of RNA in real time.

Original languageEnglish (US)
Pages (from-to)1895-1897
Number of pages3
JournalChemBioChem
Volume7
Issue number12
DOIs
StatePublished - Dec 2006
Externally publishedYes

Funding

FundersFunder number
National Institute of General Medical SciencesR01GM062536

    Keywords

    • CARS microscopy
    • Hepatitis C virus
    • Host-viral interactions
    • RNA
    • Two-photon fluorescence

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Medicine
    • Molecular Biology
    • Organic Chemistry

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