Intramembranous solute and water fluxes during high intramembranous absorption rates in fetal sheep with and without lung liquid diversion

Katherine M. Gesteland; Debra F. Anderson; Lowell E. Davis; Patricia Robertson; J. Job Faber; Robert A. Brace

doi:10.1016/j.ajog.2009.02.018

Intramembranous solute and water fluxes during high intramembranous absorption rates in fetal sheep with and without lung liquid diversion

Katherine M. Gesteland
, Debra F. Anderson
, Lowell E. Davis
, Patricia Robertson
, J. Job Faber
, Robert A. Brace

Obstetrics and Gynecology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Objective: To examine mechanisms that mediate increased intramembranous solute and water absorption. Study Design: Intramembranous solute and water fluxes were measured in fetal sheep under basal conditions and after intraamniotic infusion of lactated Ringer's solution of 4 L/d for 3 days with and without lung liquid diversion. Results: Intramembranous sodium, potassium, chloride, calcium, glucose, and lactate fluxes increased 2.5- to 7.9-fold, were linearly related to volume fluxes (r = 0.83-0.99), and were unaffected by lung liquid. All clearance rates, except that of lactate, increased to equal the intramembranous volume absorption rate during infusion. Conclusion: Under basal conditions, passive diffusion makes a minor and bulk flow a major contribution to intramembranous solute absorption. During high absorption rates, the increase in solute absorption above basal levels appears to be due entirely to bulk flow and is unaffected by lung liquid. The increased bulk flow is consistent with vesicular transcytosis.

Original language	English (US)
Pages (from-to)	85.e1-85.e6
Journal	American journal of obstetrics and gynecology
Volume	201
Issue number	1
DOIs	https://doi.org/10.1016/j.ajog.2009.02.018
State	Published - Jan 1 2009

Funding

This study was supported in part by NIH Grants 5R01HD035890, 5R01HL045043, and P01HD034430.

Funders	Funder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	5R01HL045043, P01HD034430
The National Institute of Child Health and Human Development	R01HD035890

Keywords

bulk flow
fetal sheep
intramembranous water and solute absorption
passive diffusion
vesicular transport

ASJC Scopus subject areas

Obstetrics and Gynecology

Access to Document

10.1016/j.ajog.2009.02.018

Cite this

Intramembranous solute and water fluxes during high intramembranous absorption rates in fetal sheep with and without lung liquid diversion. / Gesteland, Katherine M.; Anderson, Debra F.; Davis, Lowell E. et al.
In: American journal of obstetrics and gynecology, Vol. 201, No. 1, 01.01.2009, p. 85.e1-85.e6.

Research output: Contribution to journal › Article › peer-review

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abstract = "Objective: To examine mechanisms that mediate increased intramembranous solute and water absorption. Study Design: Intramembranous solute and water fluxes were measured in fetal sheep under basal conditions and after intraamniotic infusion of lactated Ringer's solution of 4 L/d for 3 days with and without lung liquid diversion. Results: Intramembranous sodium, potassium, chloride, calcium, glucose, and lactate fluxes increased 2.5- to 7.9-fold, were linearly related to volume fluxes (r = 0.83-0.99), and were unaffected by lung liquid. All clearance rates, except that of lactate, increased to equal the intramembranous volume absorption rate during infusion. Conclusion: Under basal conditions, passive diffusion makes a minor and bulk flow a major contribution to intramembranous solute absorption. During high absorption rates, the increase in solute absorption above basal levels appears to be due entirely to bulk flow and is unaffected by lung liquid. The increased bulk flow is consistent with vesicular transcytosis.",

keywords = "bulk flow, fetal sheep, intramembranous water and solute absorption, passive diffusion, vesicular transport",

author = "Gesteland, \{Katherine M.\} and Anderson, \{Debra F.\} and Davis, \{Lowell E.\} and Patricia Robertson and Faber, \{J. Job\} and Brace, \{Robert A.\}",

note = "Funding Information: This study was supported in part by NIH Grants 5R01HD035890, 5R01HL045043, and P01HD034430. ",

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AU - Gesteland, Katherine M.

AU - Anderson, Debra F.

AU - Davis, Lowell E.

AU - Robertson, Patricia

AU - Faber, J. Job

AU - Brace, Robert A.

N1 - Funding Information: This study was supported in part by NIH Grants 5R01HD035890, 5R01HL045043, and P01HD034430.

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N2 - Objective: To examine mechanisms that mediate increased intramembranous solute and water absorption. Study Design: Intramembranous solute and water fluxes were measured in fetal sheep under basal conditions and after intraamniotic infusion of lactated Ringer's solution of 4 L/d for 3 days with and without lung liquid diversion. Results: Intramembranous sodium, potassium, chloride, calcium, glucose, and lactate fluxes increased 2.5- to 7.9-fold, were linearly related to volume fluxes (r = 0.83-0.99), and were unaffected by lung liquid. All clearance rates, except that of lactate, increased to equal the intramembranous volume absorption rate during infusion. Conclusion: Under basal conditions, passive diffusion makes a minor and bulk flow a major contribution to intramembranous solute absorption. During high absorption rates, the increase in solute absorption above basal levels appears to be due entirely to bulk flow and is unaffected by lung liquid. The increased bulk flow is consistent with vesicular transcytosis.

AB - Objective: To examine mechanisms that mediate increased intramembranous solute and water absorption. Study Design: Intramembranous solute and water fluxes were measured in fetal sheep under basal conditions and after intraamniotic infusion of lactated Ringer's solution of 4 L/d for 3 days with and without lung liquid diversion. Results: Intramembranous sodium, potassium, chloride, calcium, glucose, and lactate fluxes increased 2.5- to 7.9-fold, were linearly related to volume fluxes (r = 0.83-0.99), and were unaffected by lung liquid. All clearance rates, except that of lactate, increased to equal the intramembranous volume absorption rate during infusion. Conclusion: Under basal conditions, passive diffusion makes a minor and bulk flow a major contribution to intramembranous solute absorption. During high absorption rates, the increase in solute absorption above basal levels appears to be due entirely to bulk flow and is unaffected by lung liquid. The increased bulk flow is consistent with vesicular transcytosis.

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KW - intramembranous water and solute absorption

KW - passive diffusion

KW - vesicular transport

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Intramembranous solute and water fluxes during high intramembranous absorption rates in fetal sheep with and without lung liquid diversion

Abstract

Funding

Keywords

ASJC Scopus subject areas

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