Inward Rectification (Ih) in Immunocytochemically‐ldentified Vasopressin and Oxytocin Neurons of Guinea‐Pig Supraoptic Nucleus

Kenneth R. Erickson; Oline K. Ronnekleiv; Martin J. Kelly

doi:10.1111/j.1365-2826.1990.tb00402.x

Inward Rectification (I_h) in Immunocytochemically‐ldentified Vasopressin and Oxytocin Neurons of Guinea‐Pig Supraoptic Nucleus

Kenneth R. Erickson
, Oline K. Ronnekleiv
, Martin J. Kelly

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Intracellular recordings of magnocellular neurons from the supraoptic nucleus of guinea‐pigs were made with KCI/K citrate‐ and biocytin‐filled electrodes. Fifty of 99 cells exhibited a time‐dependent inward rectification (TDR). The TDR was activated during hyperpolarizing current pulses to membrane potentials more hyperpolarized than −75 mV. In voltage‐clamp recordings, an inward current appeared at voltage steps more hyperpolarized than −75 mV, with properties similar to the slow inward rectifier (I_h) described in other tissues. The I_h was blocked by 2 mM CsCI. BaCI₂ (100 to 500 μM) did not block the I_h. Immunocytochemical identification of the recorded cells revealed that both vasopressin (AVP)‐ and oxytocin (OT)‐ containing neurons exhibited an I_h.

Original language	English (US)
Pages (from-to)	261-265
Number of pages	5
Journal	Journal of Neuroendocrinology
Volume	2
Issue number	3
DOIs	https://doi.org/10.1111/j.1365-2826.1990.tb00402.x
State	Published - Jun 1990

Keywords

immunocytochemistry
inward rectification
oxytocin
supraoptic nucleus
vasopressin

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology
Endocrine and Autonomic Systems
Cellular and Molecular Neuroscience

Access to Document

10.1111/j.1365-2826.1990.tb00402.x

Cite this

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title = "Inward Rectification (Ih) in Immunocytochemically‐ldentified Vasopressin and Oxytocin Neurons of Guinea‐Pig Supraoptic Nucleus",

abstract = "Intracellular recordings of magnocellular neurons from the supraoptic nucleus of guinea‐pigs were made with KCI/K citrate‐ and biocytin‐filled electrodes. Fifty of 99 cells exhibited a time‐dependent inward rectification (TDR). The TDR was activated during hyperpolarizing current pulses to membrane potentials more hyperpolarized than −75 mV. In voltage‐clamp recordings, an inward current appeared at voltage steps more hyperpolarized than −75 mV, with properties similar to the slow inward rectifier (Ih) described in other tissues. The Ih was blocked by 2 mM CsCI. BaCI2 (100 to 500 μM) did not block the Ih. Immunocytochemical identification of the recorded cells revealed that both vasopressin (AVP)‐ and oxytocin (OT)‐ containing neurons exhibited an Ih.",

keywords = "immunocytochemistry, inward rectification, oxytocin, supraoptic nucleus, vasopressin",

author = "Erickson, \{Kenneth R.\} and Ronnekleiv, \{Oline K.\} and Kelly, \{Martin J.\}",

year = "1990",

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T1 - Inward Rectification (Ih) in Immunocytochemically‐ldentified Vasopressin and Oxytocin Neurons of Guinea‐Pig Supraoptic Nucleus

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AU - Ronnekleiv, Oline K.

AU - Kelly, Martin J.

PY - 1990/6

Y1 - 1990/6

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AB - Intracellular recordings of magnocellular neurons from the supraoptic nucleus of guinea‐pigs were made with KCI/K citrate‐ and biocytin‐filled electrodes. Fifty of 99 cells exhibited a time‐dependent inward rectification (TDR). The TDR was activated during hyperpolarizing current pulses to membrane potentials more hyperpolarized than −75 mV. In voltage‐clamp recordings, an inward current appeared at voltage steps more hyperpolarized than −75 mV, with properties similar to the slow inward rectifier (Ih) described in other tissues. The Ih was blocked by 2 mM CsCI. BaCI2 (100 to 500 μM) did not block the Ih. Immunocytochemical identification of the recorded cells revealed that both vasopressin (AVP)‐ and oxytocin (OT)‐ containing neurons exhibited an Ih.

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KW - inward rectification

KW - oxytocin

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