Isolation and characterization of novel rhesus monkey embryonic stem cell lines

Shoukhrat Mitalipov, Hung Chih Kuo, James Byrne, Lisa Clepper, Lorraine Meisner, Julie Johnson, Renee Zeier, Don Wolf

    Research output: Contribution to journalArticlepeer-review

    84 Scopus citations


    ESCs are important as research subjects since the mechanisms underlying cellular differentiation, expansion, and self-renewal can be studied along with differentiated tissue development and regeneration in vitro. Furthermore, human ESCs hold promise for cell and tissue replacement approaches to treating human diseases. The rhesus monkey is a clinically relevant primate model that will likely be required to bring these clinical applications to fruition. Monkey ESCs share a number of properties with human ESCs, and their derivation and use are not affected by bioethical concerns. Here, we summarize our experience in the establishment of 18 ESC lines from rhesus monkey preimplantation embryos generated by the application of the assisted reproductive technologies. The newly derived monkey ESC lines were maintained in vitro without losing their chromosomal integrity, and they expressed markers previously reported present in human and monkey ESCs. We also describe initial efforts to compare the pluripotency of ESC lines by expression profiling, chimeric embryo formation, and in vitro-directed differentiation into endodermal, mesodermal, and ectodermal lineages.

    Original languageEnglish (US)
    Pages (from-to)2177-2186
    Number of pages10
    JournalStem Cells
    Issue number10
    StatePublished - Oct 2006


    • Chimera
    • Embryonic stem cells
    • Karyotype
    • Rhesus monkey
    • Transcriptome

    ASJC Scopus subject areas

    • Molecular Medicine
    • Developmental Biology
    • Cell Biology


    Dive into the research topics of 'Isolation and characterization of novel rhesus monkey embryonic stem cell lines'. Together they form a unique fingerprint.

    Cite this