TY - JOUR
T1 - Isolation and characterization of novel rhesus monkey embryonic stem cell lines
AU - Mitalipov, Shoukhrat
AU - Kuo, Hung Chih
AU - Byrne, James
AU - Clepper, Lisa
AU - Meisner, Lorraine
AU - Johnson, Julie
AU - Zeier, Renee
AU - Wolf, Don
N1 - Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2006/10
Y1 - 2006/10
N2 - ESCs are important as research subjects since the mechanisms underlying cellular differentiation, expansion, and self-renewal can be studied along with differentiated tissue development and regeneration in vitro. Furthermore, human ESCs hold promise for cell and tissue replacement approaches to treating human diseases. The rhesus monkey is a clinically relevant primate model that will likely be required to bring these clinical applications to fruition. Monkey ESCs share a number of properties with human ESCs, and their derivation and use are not affected by bioethical concerns. Here, we summarize our experience in the establishment of 18 ESC lines from rhesus monkey preimplantation embryos generated by the application of the assisted reproductive technologies. The newly derived monkey ESC lines were maintained in vitro without losing their chromosomal integrity, and they expressed markers previously reported present in human and monkey ESCs. We also describe initial efforts to compare the pluripotency of ESC lines by expression profiling, chimeric embryo formation, and in vitro-directed differentiation into endodermal, mesodermal, and ectodermal lineages.
AB - ESCs are important as research subjects since the mechanisms underlying cellular differentiation, expansion, and self-renewal can be studied along with differentiated tissue development and regeneration in vitro. Furthermore, human ESCs hold promise for cell and tissue replacement approaches to treating human diseases. The rhesus monkey is a clinically relevant primate model that will likely be required to bring these clinical applications to fruition. Monkey ESCs share a number of properties with human ESCs, and their derivation and use are not affected by bioethical concerns. Here, we summarize our experience in the establishment of 18 ESC lines from rhesus monkey preimplantation embryos generated by the application of the assisted reproductive technologies. The newly derived monkey ESC lines were maintained in vitro without losing their chromosomal integrity, and they expressed markers previously reported present in human and monkey ESCs. We also describe initial efforts to compare the pluripotency of ESC lines by expression profiling, chimeric embryo formation, and in vitro-directed differentiation into endodermal, mesodermal, and ectodermal lineages.
KW - Chimera
KW - Embryonic stem cells
KW - Karyotype
KW - Rhesus monkey
KW - Transcriptome
UR - http://www.scopus.com/inward/record.url?scp=33749508317&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33749508317&partnerID=8YFLogxK
U2 - 10.1634/stemcells.2006-0125
DO - 10.1634/stemcells.2006-0125
M3 - Article
C2 - 16741224
AN - SCOPUS:33749508317
SN - 1066-5099
VL - 24
SP - 2177
EP - 2186
JO - Stem Cells
JF - Stem Cells
IS - 10
ER -