Laboratory Diagnostics for Fungal Infections: A Review of Current and Future Diagnostic Assays

This article reviews the current diagnostic approaches, both serologic and molecular, for the detection of fungi associated with pulmonary disease. Classic serologic techniques, including immunodiffusion and complement fixation, both of which remain a cornerstone for fungal diagnostic testing, are reviewed and their performance characteristics presented. More recent advances in this field, including novel lateral-flow assays for fungal antigen detection, are also described. Molecular techniques for fungal identification both from culture and directly from patient specimens, including nucleic acid probes, mass spectrometry–based methods, nucleic acid amplification testing, and traditional and broad-range sequencing, are discussed and their performance evaluated.