Lentiviral-mediated transgene expression can potentiate intestinal mesenchymal-epithelial signaling

Adria D. Dismuke; Aimee D. Kohn; Randall T. Moon; Melissa H. Wong

doi:10.1007/s12575-009-9014-z

Lentiviral-mediated transgene expression can potentiate intestinal mesenchymal-epithelial signaling

Adria D. Dismuke
, Aimee D. Kohn
, Randall T. Moon
, Melissa H. Wong

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Mesenchymal-epithelial signaling is essential for the development of many organs and is often disrupted in disease. In this study, we demonstrate the use of lentiviral-mediated transgene delivery as an effective approach for ectopic transgene expression and an alternative to generation of transgenic animals. One benefit to this approach is that it can be used independently or in conjunction with established transgenic or knockout animals for studying modulation of mesenchymal-epithelial interactions. To display the power of this approach, we explored ectopic expression of a Wnt ligand in the mouse intestinal mesenchyme and demonstrate its functional influence on the adjacent epithelium. Our findings highlight the efficient use of lentiviral-mediated transgene expression for modulating mesenchymal-epithelial interactions in vivo.

Original language	English (US)
Pages (from-to)	130-144
Number of pages	15
Journal	Biological Procedures Online
Volume	11
Issue number	1
DOIs	https://doi.org/10.1007/s12575-009-9014-z
State	Published - 2009

Funding

We thank members of the Wong laboratory for helpful discussions; critical reading of the manuscript: Paige Davies, Anne Powell, Trevor Levin, John Swain, Christine Glynn; expert technical assistance with flow cytometry analysis: Anne Powell; and technical assistance with mice: Christine Glynn. This work was supported by grants from the National Institutes of Health (NIH) to M.H.W. NIH R01DK068326 and a fellowship to A.D.D. T32GM009617. R.T.M. is a Howard Hughes investigator.

Funders	Funder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	R01DK068326, T32GM009617

Keywords

Intestine
Lentivirus
Mesenchymal-epithelial signaling
Mouse
Transgene expression
Wnt signaling

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1007/s12575-009-9014-z

Cite this

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title = "Lentiviral-mediated transgene expression can potentiate intestinal mesenchymal-epithelial signaling",

abstract = "Mesenchymal-epithelial signaling is essential for the development of many organs and is often disrupted in disease. In this study, we demonstrate the use of lentiviral-mediated transgene delivery as an effective approach for ectopic transgene expression and an alternative to generation of transgenic animals. One benefit to this approach is that it can be used independently or in conjunction with established transgenic or knockout animals for studying modulation of mesenchymal-epithelial interactions. To display the power of this approach, we explored ectopic expression of a Wnt ligand in the mouse intestinal mesenchyme and demonstrate its functional influence on the adjacent epithelium. Our findings highlight the efficient use of lentiviral-mediated transgene expression for modulating mesenchymal-epithelial interactions in vivo.",

keywords = "Intestine, Lentivirus, Mesenchymal-epithelial signaling, Mouse, Transgene expression, Wnt signaling",

author = "Dismuke, \{Adria D.\} and Kohn, \{Aimee D.\} and Moon, \{Randall T.\} and Wong, \{Melissa H.\}",

note = "Funding Information: We thank members of the Wong laboratory for helpful discussions; critical reading of the manuscript: Paige Davies, Anne Powell, Trevor Levin, John Swain, Christine Glynn; expert technical assistance with flow cytometry analysis: Anne Powell; and technical assistance with mice: Christine Glynn. This work was supported by grants from the National Institutes of Health (NIH) to M.H.W. NIH R01DK068326 and a fellowship to A.D.D. T32GM009617. R.T.M. is a Howard Hughes investigator.",

year = "2009",

doi = "10.1007/s12575-009-9014-z",

language = "English (US)",

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pages = "130--144",

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T1 - Lentiviral-mediated transgene expression can potentiate intestinal mesenchymal-epithelial signaling

AU - Dismuke, Adria D.

AU - Kohn, Aimee D.

AU - Moon, Randall T.

AU - Wong, Melissa H.

N1 - Funding Information: We thank members of the Wong laboratory for helpful discussions; critical reading of the manuscript: Paige Davies, Anne Powell, Trevor Levin, John Swain, Christine Glynn; expert technical assistance with flow cytometry analysis: Anne Powell; and technical assistance with mice: Christine Glynn. This work was supported by grants from the National Institutes of Health (NIH) to M.H.W. NIH R01DK068326 and a fellowship to A.D.D. T32GM009617. R.T.M. is a Howard Hughes investigator.

PY - 2009

Y1 - 2009

N2 - Mesenchymal-epithelial signaling is essential for the development of many organs and is often disrupted in disease. In this study, we demonstrate the use of lentiviral-mediated transgene delivery as an effective approach for ectopic transgene expression and an alternative to generation of transgenic animals. One benefit to this approach is that it can be used independently or in conjunction with established transgenic or knockout animals for studying modulation of mesenchymal-epithelial interactions. To display the power of this approach, we explored ectopic expression of a Wnt ligand in the mouse intestinal mesenchyme and demonstrate its functional influence on the adjacent epithelium. Our findings highlight the efficient use of lentiviral-mediated transgene expression for modulating mesenchymal-epithelial interactions in vivo.

AB - Mesenchymal-epithelial signaling is essential for the development of many organs and is often disrupted in disease. In this study, we demonstrate the use of lentiviral-mediated transgene delivery as an effective approach for ectopic transgene expression and an alternative to generation of transgenic animals. One benefit to this approach is that it can be used independently or in conjunction with established transgenic or knockout animals for studying modulation of mesenchymal-epithelial interactions. To display the power of this approach, we explored ectopic expression of a Wnt ligand in the mouse intestinal mesenchyme and demonstrate its functional influence on the adjacent epithelium. Our findings highlight the efficient use of lentiviral-mediated transgene expression for modulating mesenchymal-epithelial interactions in vivo.

KW - Intestine

KW - Lentivirus

KW - Mesenchymal-epithelial signaling

KW - Mouse

KW - Transgene expression

KW - Wnt signaling

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UR - https://www.scopus.com/pages/publications/77649272217#tab=citedBy

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DO - 10.1007/s12575-009-9014-z

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SN - 1480-9222

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JO - Biological Procedures Online

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IS - 1

ER -

Lentiviral-mediated transgene expression can potentiate intestinal mesenchymal-epithelial signaling

Abstract

Funding

Keywords

ASJC Scopus subject areas

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