Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution

Yichen Ding; Juhyun Lee; Jianguo Ma; Kevin Sung; Tomohiro Yokota; Neha Singh; Mojdeh Dooraghi; Parinaz Abiri; Yibin Wang; Rajan P. Kulkarni; Atsushi Nakano; Thao P. Nguyen; Peng Fei; Tzung K. Hsiai

doi:10.1038/srep42209

Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution

Yichen Ding
, Juhyun Lee
, Jianguo Ma
, Kevin Sung
, Tomohiro Yokota
, Neha Singh
, Mojdeh Dooraghi
, Parinaz Abiri
, Yibin Wang
, Rajan P. Kulkarni
, Atsushi Nakano
, Thao P. Nguyen
, Peng Fei
, Tzung K. Hsiai

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Light-sheet fluorescence microscopy (LSFM) serves to advance developmental research and regenerative medicine. Coupled with the paralleled advances in fluorescence-friendly tissue clearing technique, our cardiac LSFM enables dual-sided illumination to rapidly uncover the architecture of murine hearts over 10 by 10 by 10 mm 3 in volume; thereby allowing for localizing progenitor differentiation to the cardiomyocyte lineage and AAV9-mediated expression of exogenous transmembrane potassium channels with high contrast and resolution. Without the steps of stitching image columns, pivoting the light-sheet and sectioning the heart mechanically, we establish a holistic strategy for 3-dimentional reconstruction of the "digital murine heart" to assess aberrant cardiac structures as well as the spatial distribution of the cardiac lineages in neonates and ion-channels in adults.

Original language	English (US)
Article number	42209
Journal	Scientific Reports
Volume	7
DOIs	https://doi.org/10.1038/srep42209
State	Published - Feb 6 2017
Externally published	Yes

Funding

This work was supported by the National Institutes of Health (5R01HL083015-10, 1R01HL118650, 1R01HL129727, 7R01HL111437, U54 EB022002, P41-EB02182)

Funders	Funder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	5R01HL083015-10, U54 EB022002, P41-EB02182, 1R01HL118650, 7R01HL111437, 1R01HL129727
National Center for Advancing Translational Sciences	KL2TR001882

ASJC Scopus subject areas

General

Access to Document

10.1038/srep42209

Cite this

@article{7f71cd154284456e8291412432b27055,

title = "Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution",

abstract = "Light-sheet fluorescence microscopy (LSFM) serves to advance developmental research and regenerative medicine. Coupled with the paralleled advances in fluorescence-friendly tissue clearing technique, our cardiac LSFM enables dual-sided illumination to rapidly uncover the architecture of murine hearts over 10 by 10 by 10 mm 3 in volume; thereby allowing for localizing progenitor differentiation to the cardiomyocyte lineage and AAV9-mediated expression of exogenous transmembrane potassium channels with high contrast and resolution. Without the steps of stitching image columns, pivoting the light-sheet and sectioning the heart mechanically, we establish a holistic strategy for 3-dimentional reconstruction of the {"}digital murine heart{"} to assess aberrant cardiac structures as well as the spatial distribution of the cardiac lineages in neonates and ion-channels in adults.",

author = "Yichen Ding and Juhyun Lee and Jianguo Ma and Kevin Sung and Tomohiro Yokota and Neha Singh and Mojdeh Dooraghi and Parinaz Abiri and Yibin Wang and Kulkarni, \{Rajan P.\} and Atsushi Nakano and Nguyen, \{Thao P.\} and Peng Fei and Hsiai, \{Tzung K.\}",

note = "Funding Information: This work was supported by the National Institutes of Health (5R01HL083015-10, 1R01HL118650, 1R01HL129727, 7R01HL111437, U54 EB022002, P41-EB02182)",

year = "2017",

month = feb,

day = "6",

doi = "10.1038/srep42209",

language = "English (US)",

volume = "7",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Research",

}

TY - JOUR

T1 - Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution

AU - Ding, Yichen

AU - Lee, Juhyun

AU - Ma, Jianguo

AU - Sung, Kevin

AU - Yokota, Tomohiro

AU - Singh, Neha

AU - Dooraghi, Mojdeh

AU - Abiri, Parinaz

AU - Wang, Yibin

AU - Kulkarni, Rajan P.

AU - Nakano, Atsushi

AU - Nguyen, Thao P.

AU - Fei, Peng

AU - Hsiai, Tzung K.

N1 - Funding Information: This work was supported by the National Institutes of Health (5R01HL083015-10, 1R01HL118650, 1R01HL129727, 7R01HL111437, U54 EB022002, P41-EB02182)

PY - 2017/2/6

Y1 - 2017/2/6

N2 - Light-sheet fluorescence microscopy (LSFM) serves to advance developmental research and regenerative medicine. Coupled with the paralleled advances in fluorescence-friendly tissue clearing technique, our cardiac LSFM enables dual-sided illumination to rapidly uncover the architecture of murine hearts over 10 by 10 by 10 mm 3 in volume; thereby allowing for localizing progenitor differentiation to the cardiomyocyte lineage and AAV9-mediated expression of exogenous transmembrane potassium channels with high contrast and resolution. Without the steps of stitching image columns, pivoting the light-sheet and sectioning the heart mechanically, we establish a holistic strategy for 3-dimentional reconstruction of the "digital murine heart" to assess aberrant cardiac structures as well as the spatial distribution of the cardiac lineages in neonates and ion-channels in adults.

AB - Light-sheet fluorescence microscopy (LSFM) serves to advance developmental research and regenerative medicine. Coupled with the paralleled advances in fluorescence-friendly tissue clearing technique, our cardiac LSFM enables dual-sided illumination to rapidly uncover the architecture of murine hearts over 10 by 10 by 10 mm 3 in volume; thereby allowing for localizing progenitor differentiation to the cardiomyocyte lineage and AAV9-mediated expression of exogenous transmembrane potassium channels with high contrast and resolution. Without the steps of stitching image columns, pivoting the light-sheet and sectioning the heart mechanically, we establish a holistic strategy for 3-dimentional reconstruction of the "digital murine heart" to assess aberrant cardiac structures as well as the spatial distribution of the cardiac lineages in neonates and ion-channels in adults.

UR - https://www.scopus.com/pages/publications/85011805769

UR - https://www.scopus.com/pages/publications/85011805769#tab=citedBy

U2 - 10.1038/srep42209

DO - 10.1038/srep42209

M3 - Article

C2 - 28165052

AN - SCOPUS:85011805769

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

M1 - 42209

ER -

Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution

Abstract

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this