@article{3db9b73633b740fcbdaf8c8f2c37dc64,
title = "Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics",
abstract = "We demonstrate live-cell super-resolution imaging using photoactivated localization microscopy (PALM). The use of photon-tolerant cell lines in combination with the high resolution and molecular sensitivity of PALM permitted us to investigate the nanoscale dynamics within individual adhesion complexes (ACs) in living cells under physiological conditions for as long as 25 min, with half of the time spent collecting the PALM images at spatial resolutions down to ∼60 nm and frame rates as short as 25 s. We visualized the formation of ACs and measured the fractional gain and loss of individual paxillin molecules as each AC evolved. By allowing observation of a wide variety of nanoscale dynamics, live-cell PALM provides insights into molecular assembly during the initiation, maturation and dissolution of cellular processes.",
author = "Hari Shroff and Galbraith, {Catherine G.} and Galbraith, {James A.} and Eric Betzig",
note = "Funding Information: We thank H. White for assistance with cell culture, M. Davidson (Florida State University) for the tdEos-paxillin plasmid and H. Peng (Janelia Farm Research Campus) for creating the simulation leading to Supplementary Fig. 5. This research was supported in part by the Intramural Research Programs of the US National Institutes of Health, the National Institute of Dental and Craniofacial Research, and the National Institute of Neurological Disorders and Stroke. Funding Information: 1Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA. 2National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Drive, Bethesda, Maryland 20892, USA. 3National Institute of Neurological Disorders and Stroke, National Institutes of Health, 49 Convent Drive, Bethesda, Maryland 20892, USA. 4These authors contributed equally to this work. Correspondence should be addressed to H.S. (shroffh@janelia.hhmi.org).",
year = "2008",
month = may,
doi = "10.1038/nmeth.1202",
language = "English (US)",
volume = "5",
pages = "417--423",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "5",
}