TY - JOUR
T1 - Low CD27 expression in plasma cell dyscrasias correlates with high-risk disease
T2 - An immunohistochemical analysis
AU - Morgan, Terry K.
AU - Zhao, Shuchun
AU - Chang, Karen L.
AU - Haddix, Terri L.
AU - Domanay, Elisabeth
AU - Cornbleet, P. Joanne
AU - Arber, Daniel A.
AU - Natkunam, Yasodha
PY - 2006/10
Y1 - 2006/10
N2 - Genome-wide expression studies using complementary DNA microarrays recently suggested a number of intriguing candidate genes for distinguishing plasma cell dyscrasias. Our objective was to test select markers using immunohistochemical analysis and a tissue microarray from paraffin-embedded bone marrow core biopsy specimens obtained from 8 patients with monoclonal gammopathy of undetermined significance, 17 with plasmacytoma, 160 with multiple myeloma, and 15 with plasma cell leukemia (PCL). We immunostained serial sections for CD138, CD27, CD56, p27, Ki-67, CD3, and CD20. Each core was scored in duplicate by observers blinded to phenotype and reported as the average percentage of CD138+ cells. The Mann-Whitney U test was used to determine significance between groups. PCL showed significantly less immunostaining for CD27 (P < .01) and p27 (P < .05) compared with plasmacytoma and multiple myeloma. Low CD27 expression also was associated with plasmacytoma progression to multiple myeloma (P < .05). Our results support the hypothesis that low CD27 expression correlates with high-risk disease, including primary PCL and decreased progression-free survival in solitary plasmacytoma.
AB - Genome-wide expression studies using complementary DNA microarrays recently suggested a number of intriguing candidate genes for distinguishing plasma cell dyscrasias. Our objective was to test select markers using immunohistochemical analysis and a tissue microarray from paraffin-embedded bone marrow core biopsy specimens obtained from 8 patients with monoclonal gammopathy of undetermined significance, 17 with plasmacytoma, 160 with multiple myeloma, and 15 with plasma cell leukemia (PCL). We immunostained serial sections for CD138, CD27, CD56, p27, Ki-67, CD3, and CD20. Each core was scored in duplicate by observers blinded to phenotype and reported as the average percentage of CD138+ cells. The Mann-Whitney U test was used to determine significance between groups. PCL showed significantly less immunostaining for CD27 (P < .01) and p27 (P < .05) compared with plasmacytoma and multiple myeloma. Low CD27 expression also was associated with plasmacytoma progression to multiple myeloma (P < .05). Our results support the hypothesis that low CD27 expression correlates with high-risk disease, including primary PCL and decreased progression-free survival in solitary plasmacytoma.
KW - CD27
KW - MGUS
KW - Monoclonal gammopathy of undetermined significance
KW - Myeloma
KW - Plasma cell leukemia
KW - Plasmacytoma
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U2 - 10.1309/ELGMGX81C2UTP55R
DO - 10.1309/ELGMGX81C2UTP55R
M3 - Article
C2 - 16938662
AN - SCOPUS:33749319345
SN - 0002-9173
VL - 126
SP - 545
EP - 551
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 4
ER -