MAP2 is localized to the dendrites of hippocampal neurons which develop in culture

Alfredo Caceres; Gary Banker; Oswald Steward; Lester Binder; Michael Payne

doi:10.1016/0165-3806(84)90167-6

MAP2 is localized to the dendrites of hippocampal neurons which develop in culture

Alfredo Caceres
, Gary Banker
, Oswald Steward
, Lester Binder
, Michael Payne

Research output: Contribution to journal › Article › peer-review

294 Scopus citations

Abstract

The distribution of the microtubule-associated protein MAP2 in cultured hippocampal neurons was studied using immunocytochemistry with monoclonal antibodies. MAP2 was preferentially localized to dendritic, but not axonal, processes even in single isolated cells which developed without making intercellular contacts. Hence regional differences in the molecular composition of the neuronal cytoskeleton can develop independently of cell interactions. The presence of MAP2 may be a useful marker for identifying dendrites in cell culture.

Original language	English (US)
Pages (from-to)	314-318
Number of pages	5
Journal	Developmental Brain Research
Volume	13
Issue number	2
DOIs	https://doi.org/10.1016/0165-3806(84)90167-6
State	Published - Apr 1984
Externally published	Yes

Funding

This work was supportedb y NIH Grants NS17112 (to G.B.) and NS12333( to O.S.) and by FogartyI n-ternationaFl ellowshipF STW02910Ato A.C. who was on leavef rom the Departmenotf Neurosurgery, Universityo f Virginia. We thank Ann Lohr and Lee Snavelyfo r technicaal ssistance.

Funders	Funder number
Departmenotf Neurosurgery, Universityo f Virginia
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	NS12333
National Institute of Neurological Disorders and Stroke	R01NS017112

Keywords

cytoskeleton
dendrites
hippocampal neurons
immunocytochemistry
microtubule-associated proteins
monoclonal antibodies
neuronal development
tissue culture

ASJC Scopus subject areas

Developmental Neuroscience
Developmental Biology

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10.1016/0165-3806(84)90167-6

Cite this

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title = "MAP2 is localized to the dendrites of hippocampal neurons which develop in culture",

abstract = "The distribution of the microtubule-associated protein MAP2 in cultured hippocampal neurons was studied using immunocytochemistry with monoclonal antibodies. MAP2 was preferentially localized to dendritic, but not axonal, processes even in single isolated cells which developed without making intercellular contacts. Hence regional differences in the molecular composition of the neuronal cytoskeleton can develop independently of cell interactions. The presence of MAP2 may be a useful marker for identifying dendrites in cell culture.",

keywords = "cytoskeleton, dendrites, hippocampal neurons, immunocytochemistry, microtubule-associated proteins, monoclonal antibodies, neuronal development, tissue culture",

author = "Alfredo Caceres and Gary Banker and Oswald Steward and Lester Binder and Michael Payne",

note = "Funding Information: This work was supportedb y NIH Grants NS17112 (to G.B.) and NS12333( to O.S.) and by FogartyI n-ternationaFl ellowshipF STW02910Ato A.C. who was on leavef rom the Departmenotf Neurosurgery, Universityo f Virginia. We thank Ann Lohr and Lee Snavelyfo r technicaal ssistance.",

year = "1984",

month = apr,

doi = "10.1016/0165-3806(84)90167-6",

language = "English (US)",

volume = "13",

pages = "314--318",

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T1 - MAP2 is localized to the dendrites of hippocampal neurons which develop in culture

AU - Caceres, Alfredo

AU - Banker, Gary

AU - Steward, Oswald

AU - Binder, Lester

AU - Payne, Michael

N1 - Funding Information: This work was supportedb y NIH Grants NS17112 (to G.B.) and NS12333( to O.S.) and by FogartyI n-ternationaFl ellowshipF STW02910Ato A.C. who was on leavef rom the Departmenotf Neurosurgery, Universityo f Virginia. We thank Ann Lohr and Lee Snavelyfo r technicaal ssistance.

PY - 1984/4

Y1 - 1984/4

N2 - The distribution of the microtubule-associated protein MAP2 in cultured hippocampal neurons was studied using immunocytochemistry with monoclonal antibodies. MAP2 was preferentially localized to dendritic, but not axonal, processes even in single isolated cells which developed without making intercellular contacts. Hence regional differences in the molecular composition of the neuronal cytoskeleton can develop independently of cell interactions. The presence of MAP2 may be a useful marker for identifying dendrites in cell culture.

AB - The distribution of the microtubule-associated protein MAP2 in cultured hippocampal neurons was studied using immunocytochemistry with monoclonal antibodies. MAP2 was preferentially localized to dendritic, but not axonal, processes even in single isolated cells which developed without making intercellular contacts. Hence regional differences in the molecular composition of the neuronal cytoskeleton can develop independently of cell interactions. The presence of MAP2 may be a useful marker for identifying dendrites in cell culture.

KW - cytoskeleton

KW - dendrites

KW - hippocampal neurons

KW - immunocytochemistry

KW - microtubule-associated proteins

KW - monoclonal antibodies

KW - neuronal development

KW - tissue culture

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DO - 10.1016/0165-3806(84)90167-6

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AN - SCOPUS:0021418148

SN - 0165-3806

VL - 13

SP - 314

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JO - Developmental Brain Research

JF - Developmental Brain Research

IS - 2

ER -

MAP2 is localized to the dendrites of hippocampal neurons which develop in culture

Abstract

Funding

Keywords

ASJC Scopus subject areas

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