Measurement and purification of human chromosomes by flow cytometry and sorting

A. V. Carrano, J. W. Gray, R. G. Langlois, K. J. Burkhart-Schultz, M. A. Van Dilla

Research output: Contribution to journalArticlepeer-review

95 Scopus citations


The 24 human chromosome types of a normal diploid fibroblast cell strain were classified into 15 groups by high-resolution flow cytometry on the basis of 33258 Hoechst fluorescence. Chromosomes associated with each group were flow sorted onto microscope slides and identified by quinacrine banding analysis. DNA cytophotometry of metaphase chromosomes from the same cell strain supported and extended this identification. Four of the groups purified were due to chromosomes of a single type - namely, chromosomes 5, 6, 13, and 17. Eight additional groups were also separated and found to contain the following chromosomes: 1 and 2; 3 and 4; 7, 8, and X; 9-12; 14 and 15; 16 and 18; 20 and Y; and 19, 21, and 22. The average purity for the 12 sorted fractions was 78%.

Original languageEnglish (US)
Pages (from-to)1382-1384
Number of pages3
JournalUnknown Journal
Issue number3
StatePublished - 1979
Externally publishedYes

ASJC Scopus subject areas

  • General


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