TY - JOUR
T1 - Measurement of DNA antibodies
AU - Bennett, R. M.
AU - Molina, E.
PY - 1976/1/1
Y1 - 1976/1/1
N2 - Modifications of the standard Farr technique for assaying DNA antibodies are presented; these result in improved separation of normals from abnormals, enhanced reproducibility, and simplicity of performance. The use of a 0.4 M borate buffer extends the range of the assay, while a 0.1 M buffer aids differentiation when equivocal results are obtained. Centrifugation of vials prior to counting improves the reproducibility by approximately 3%. Polyethylene glycol, at a final concentration of 6 Gm. per 100 ml., can be substituted for ammonium sulfate, with some advantages.
AB - Modifications of the standard Farr technique for assaying DNA antibodies are presented; these result in improved separation of normals from abnormals, enhanced reproducibility, and simplicity of performance. The use of a 0.4 M borate buffer extends the range of the assay, while a 0.1 M buffer aids differentiation when equivocal results are obtained. Centrifugation of vials prior to counting improves the reproducibility by approximately 3%. Polyethylene glycol, at a final concentration of 6 Gm. per 100 ml., can be substituted for ammonium sulfate, with some advantages.
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U2 - 10.1093/ajcp/65.3.364
DO - 10.1093/ajcp/65.3.364
M3 - Article
C2 - 1258828
AN - SCOPUS:0017256014
SN - 0002-9173
VL - 65
SP - 364
EP - 367
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 3
ER -