Measurement of mitochondrial respiration in trophoblast culture

A. Maloyan; J. Mele; B. Muralimanohara; L. Myatt

doi:10.1016/j.placenta.2012.01.016

Measurement of mitochondrial respiration in trophoblast culture

A. Maloyan, J. Mele, B. Muralimanohara, L. Myatt

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Pregnancy is a state of oxidative stress, which becomes exaggerated under pathological conditions, such as preeclampsia, IUGR, diabetes and obesity, where placental mitochondrial dysfunction is observed. The majority of investigations utilize isolated mitochondria when measuring mitochondrial activity in placenta. However, this does not provide a complete physiological readout of mitochondrial function. This technical note describes a method to measure respiratory function in intact primary syncytiotrophoblast from human term placenta.

Original language	English (US)
Pages (from-to)	456-458
Number of pages	3
Journal	Placenta
Volume	33
Issue number	5
DOIs	https://doi.org/10.1016/j.placenta.2012.01.016
State	Published - May 2012
Externally published	Yes

Keywords

Hypoxia
Mitochondria
Oxygen consumption
Placenta
Trophoblast

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology
Developmental Biology

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10.1016/j.placenta.2012.01.016

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abstract = "Pregnancy is a state of oxidative stress, which becomes exaggerated under pathological conditions, such as preeclampsia, IUGR, diabetes and obesity, where placental mitochondrial dysfunction is observed. The majority of investigations utilize isolated mitochondria when measuring mitochondrial activity in placenta. However, this does not provide a complete physiological readout of mitochondrial function. This technical note describes a method to measure respiratory function in intact primary syncytiotrophoblast from human term placenta.",

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AU - Maloyan, A.

AU - Mele, J.

AU - Muralimanohara, B.

AU - Myatt, L.

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N2 - Pregnancy is a state of oxidative stress, which becomes exaggerated under pathological conditions, such as preeclampsia, IUGR, diabetes and obesity, where placental mitochondrial dysfunction is observed. The majority of investigations utilize isolated mitochondria when measuring mitochondrial activity in placenta. However, this does not provide a complete physiological readout of mitochondrial function. This technical note describes a method to measure respiratory function in intact primary syncytiotrophoblast from human term placenta.

AB - Pregnancy is a state of oxidative stress, which becomes exaggerated under pathological conditions, such as preeclampsia, IUGR, diabetes and obesity, where placental mitochondrial dysfunction is observed. The majority of investigations utilize isolated mitochondria when measuring mitochondrial activity in placenta. However, this does not provide a complete physiological readout of mitochondrial function. This technical note describes a method to measure respiratory function in intact primary syncytiotrophoblast from human term placenta.

KW - Hypoxia

KW - Mitochondria

KW - Oxygen consumption

KW - Placenta

KW - Trophoblast

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JO - Placenta

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ER -

Measurement of mitochondrial respiration in trophoblast culture

Abstract

Keywords

ASJC Scopus subject areas

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