Mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome (hemopoietic dysplasia)

Grover C. Bagby

doi:10.1016/0145-2126(80)90068-5

Mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome (hemopoietic dysplasia)

Grover C. Bagby

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

We have analyzed mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome by culturing in agar and methylcellulose bone marrow cells from 5 glucocorticosteroid-responsive patients with the preleukemic syndrome. We have found that methylprednisolone and cortisol are equally effective in enhancing colony growth in these patients, that enhancement occurs in either methylcellulose or agar matrix and in RPMI 1640, McCoy's 5a, and alpha medium. In 4 patients, glucocorticosteroids inhibited the activity of T-lymphocyte inhibitors of granulopoiesis in vitro. In one patient, these agents stimulated the immediate proliferation of CFU-C and in the presence of LCM enhanced CFU-C survival in suspension culture. These effects were blocked by progesterone and not due to enhancement of CSA production or inhibition of inhibitory T-cell function. Our results document the involvement of cortisol sensitive T-lymphocytes in the suppression of granulopoiesis in a small number of patients with the preleukemic syndrome and indicate that the mechanisms of glucocorticosteroid responses among patients with this syndrome are heterogeneous.

Original language	English (US)
Pages (from-to)	571-580
Number of pages	10
Journal	Leukemia Research
Volume	4
Issue number	6
DOIs	https://doi.org/10.1016/0145-2126(80)90068-5
State	Published - 1980
Externally published	Yes

Funding

Abbreviations: CSA, Colony stimulating activity; LCM, Leukocyte conditioned medium; CFU-C, Granulocyte/macrophage colony forming units; T-cells, Thymus dependent lymphocytes; LDBMC, Light density bone marrow cells; ARA-C, Cytosine arabinoside; SRBC, Sheep red blood cells. Corres~ to: Grover C. Bagby, Jr., M.D., c/o Ann Irwin, Leukemia Research, V.A. Medical Center, Portland, OR 97207, U.S.A. *Supported by the Medical Research Service of the U.S. Veterans Administration, Portland, Oregon. "l'With Technical Assistance of Brenda Wilkinson.

Funders
Medical Research Service of the Veterans Administration and United States Public Health Service
V.A. Medical Center
U.S. Department of Veterans Affairs

Keywords

CFU-C
Glucocorticosteroids
T-lymphocytes
preleukemic syndrome

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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10.1016/0145-2126(80)90068-5

Cite this

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title = "Mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome (hemopoietic dysplasia)",

abstract = "We have analyzed mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome by culturing in agar and methylcellulose bone marrow cells from 5 glucocorticosteroid-responsive patients with the preleukemic syndrome. We have found that methylprednisolone and cortisol are equally effective in enhancing colony growth in these patients, that enhancement occurs in either methylcellulose or agar matrix and in RPMI 1640, McCoy's 5a, and alpha medium. In 4 patients, glucocorticosteroids inhibited the activity of T-lymphocyte inhibitors of granulopoiesis in vitro. In one patient, these agents stimulated the immediate proliferation of CFU-C and in the presence of LCM enhanced CFU-C survival in suspension culture. These effects were blocked by progesterone and not due to enhancement of CSA production or inhibition of inhibitory T-cell function. Our results document the involvement of cortisol sensitive T-lymphocytes in the suppression of granulopoiesis in a small number of patients with the preleukemic syndrome and indicate that the mechanisms of glucocorticosteroid responses among patients with this syndrome are heterogeneous.",

keywords = "CFU-C, Glucocorticosteroids, T-lymphocytes, preleukemic syndrome",

author = "Bagby, \{Grover C.\}",

note = "Funding Information: Abbreviations: CSA, Colony stimulating activity; LCM, Leukocyte conditioned medium; CFU-C, Granulocyte/macrophage colony forming units; T-cells, Thymus dependent lymphocytes; LDBMC, Light density bone marrow cells; ARA-C, Cytosine arabinoside; SRBC, Sheep red blood cells. Corres\textasciitilde{} to: Grover C. Bagby, Jr., M.D., c/o Ann Irwin, Leukemia Research, V.A. Medical Center, Portland, OR 97207, U.S.A. *Supported by the Medical Research Service of the U.S. Veterans Administration, Portland, Oregon. {"}l'With Technical Assistance of Brenda Wilkinson.",

year = "1980",

doi = "10.1016/0145-2126(80)90068-5",

language = "English (US)",

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pages = "571--580",

journal = "Leukemia Research",

issn = "0145-2126",

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T1 - Mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome (hemopoietic dysplasia)

AU - Bagby, Grover C.

N1 - Funding Information: Abbreviations: CSA, Colony stimulating activity; LCM, Leukocyte conditioned medium; CFU-C, Granulocyte/macrophage colony forming units; T-cells, Thymus dependent lymphocytes; LDBMC, Light density bone marrow cells; ARA-C, Cytosine arabinoside; SRBC, Sheep red blood cells. Corres~ to: Grover C. Bagby, Jr., M.D., c/o Ann Irwin, Leukemia Research, V.A. Medical Center, Portland, OR 97207, U.S.A. *Supported by the Medical Research Service of the U.S. Veterans Administration, Portland, Oregon. "l'With Technical Assistance of Brenda Wilkinson.

PY - 1980

Y1 - 1980

N2 - We have analyzed mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome by culturing in agar and methylcellulose bone marrow cells from 5 glucocorticosteroid-responsive patients with the preleukemic syndrome. We have found that methylprednisolone and cortisol are equally effective in enhancing colony growth in these patients, that enhancement occurs in either methylcellulose or agar matrix and in RPMI 1640, McCoy's 5a, and alpha medium. In 4 patients, glucocorticosteroids inhibited the activity of T-lymphocyte inhibitors of granulopoiesis in vitro. In one patient, these agents stimulated the immediate proliferation of CFU-C and in the presence of LCM enhanced CFU-C survival in suspension culture. These effects were blocked by progesterone and not due to enhancement of CSA production or inhibition of inhibitory T-cell function. Our results document the involvement of cortisol sensitive T-lymphocytes in the suppression of granulopoiesis in a small number of patients with the preleukemic syndrome and indicate that the mechanisms of glucocorticosteroid responses among patients with this syndrome are heterogeneous.

AB - We have analyzed mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome by culturing in agar and methylcellulose bone marrow cells from 5 glucocorticosteroid-responsive patients with the preleukemic syndrome. We have found that methylprednisolone and cortisol are equally effective in enhancing colony growth in these patients, that enhancement occurs in either methylcellulose or agar matrix and in RPMI 1640, McCoy's 5a, and alpha medium. In 4 patients, glucocorticosteroids inhibited the activity of T-lymphocyte inhibitors of granulopoiesis in vitro. In one patient, these agents stimulated the immediate proliferation of CFU-C and in the presence of LCM enhanced CFU-C survival in suspension culture. These effects were blocked by progesterone and not due to enhancement of CSA production or inhibition of inhibitory T-cell function. Our results document the involvement of cortisol sensitive T-lymphocytes in the suppression of granulopoiesis in a small number of patients with the preleukemic syndrome and indicate that the mechanisms of glucocorticosteroid responses among patients with this syndrome are heterogeneous.

KW - CFU-C

KW - Glucocorticosteroids

KW - T-lymphocytes

KW - preleukemic syndrome

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ER -

Mechanisms of glucocorticosteroid activity in patients with the preleukemic syndrome (hemopoietic dysplasia)

Abstract

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Keywords

ASJC Scopus subject areas

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