TY - JOUR
T1 - Membrane-bound FRET probe visualizes MMP12 activity in pulmonary inflammation
AU - Cobos-Correa, Amanda
AU - Trojanek, Johanna B.
AU - Diemer, Stefanie
AU - Mall, Marcus A.
AU - Schultz, Carsten
N1 - Funding Information:
We thank M. Gutierrez (EMBL) for RAW 264.7 macrophages, O. Gallego (EMBL) for liposomes, H. Stichnoth (EMBL) for tissue culture, S. Hirtz for genotyping, S. Schubert for discussions and mouse work and J.R. Harkema (Michigan State University) for providing particulate matter (PM10). This work was supported in part by grants from the European Union (LSHG-CT-2003-503259 to C.S. and MEXT-2004-013666 to M.A.M.).
PY - 2009/9
Y1 - 2009/9
N2 - MMP12 is a metalloproteinase implicated in inflammation. To monitor its activity, we synthesized a membrane-targeted reporter (LaRee1) based on Foerster resonance energy transfer (FRET). Unlike existing sensors, LaRee1 detects MMP12 activity by loss of FRET plus internalization of the lipidated fragment. In bronchoalveolar lavages from a mouse model of pulmonary inflammation, LaRee1 detected MMP12 activity at the surface of activated macrophages. LaRee1 may become a powerful tool for monitoring lung disease.
AB - MMP12 is a metalloproteinase implicated in inflammation. To monitor its activity, we synthesized a membrane-targeted reporter (LaRee1) based on Foerster resonance energy transfer (FRET). Unlike existing sensors, LaRee1 detects MMP12 activity by loss of FRET plus internalization of the lipidated fragment. In bronchoalveolar lavages from a mouse model of pulmonary inflammation, LaRee1 detected MMP12 activity at the surface of activated macrophages. LaRee1 may become a powerful tool for monitoring lung disease.
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U2 - 10.1038/nchembio.196
DO - 10.1038/nchembio.196
M3 - Article
C2 - 19648933
AN - SCOPUS:69249231952
SN - 1552-4450
VL - 5
SP - 628
EP - 630
JO - Nature Chemical Biology
JF - Nature Chemical Biology
IS - 9
ER -