@article{47ab6f62f4a940879f553371c4c09c4e,
title = "Methamphetamine use alters human plasma extracellular vesicles and their microRNA cargo: An exploratory study",
abstract = "Methamphetamine (MA) is the largest drug threat across the globe, with health effects including neurotoxicity and cardiovascular disease. Recent studies have begun to link microRNAs (miRNAs) to the processes related to MA use and addiction. Our studies are the first to analyse plasma EVs and their miRNA cargo in humans actively using MA (MA-ACT) and control participants (CTL). In this cohort we also assessed the effects of tobacco use on plasma EVs. We used vesicle flow cytometry to show that the MA-ACT group had an increased abundance of EV tetraspanin markers (CD9, CD63, CD81), but not pro-coagulant, platelet-, and red blood cell-derived EVs. We also found that of the 169 plasma EV miRNAs, eight were of interest in MA-ACT based on multiple statistical criteria. In smokers, we identified 15 miRNAs of interest, two that overlapped with the eight MA-ACT miRNAs. Three of the MA-ACT miRNAs significantly correlated with clinical features of MA use and target prediction with these miRNAs identified pathways implicated in MA use, including cardiovascular disease and neuroinflammation. Together our findings indicate that MA use regulates EVs and their miRNA cargo, and support that further studies are warranted to investigate their mechanistic role in addiction, recovery, and recidivism.",
keywords = "addiction, extracellular vesicle, methamphetamine, microRNA, plasma, tobacco, vesicle flow cytometry",
author = "Sandau, {Ursula S.} and Erika Duggan and Xiao Shi and Smith, {Sierra J.} and Marilyn Huckans and Schutzer, {William E.} and Loftis, {Jennifer M.} and Aaron Janowsky and Nolan, {John P.} and Saugstad, {Julie A.}",
note = "Funding Information: The authors would like to thank the study participants and staff at each of the recruitment sites, including Central City Concern, CODA, Inc., De Paul Treatment Centers, Native American Rehabilitation Association of the Northwest, OutsideIn, Volunteers of America Treatment Centers, OHSU, and the Veterans Affairs Portland Health Care System Mental Health Division and Substance Abuse Treatment Program. The authors are also grateful to Alissa Bazinet, Ph.D., Matthew Arbuckle, B.S., and Bethany Winters, B.S. for their many contributions to this project as Research Assistants and Psychometrists at the MARC. The authors would also like to acknowledge the OHSU Gene Profiling Shared Resource for providing support for the qPCR assays, under the direction of Christina A. Harrington, PhD. Consultation for the analysis of the qPCR data was provided by Jack Wiedrick, M.S. at the OHSU Biostatistics & Design Program. Transmission electron microscopy was performed at the Multiscale Microscopy Core with technical support from the OHSU‐FEI Living Lab and the OHSU Center for Spatial Systems Biomedicine, under the guidance of Claudia S. Lopez, PhD. The work was conducted using facilities at the VA Portland Health Care System and OHSU, Portland, Oregon. Authors JML (Research Scientist), AJ (Senior Research Career Scientist), and MH (Staff Psychologist and Neuropsychologist) acknowledge their appointments at the VA Portland Health Care System, Portland, Oregon. The contents do not represent the views of the Department of Veterans Affairs or the United States Government. This work was supported by the MARC under grant P50 DA018165 and Department of Veterans Affairs, Veterans Health Administration, Office of Research and Development, Biomedical Laboratory Research and Development Merit Review Awards [1I01BX002061 (JML), I01BX004934 (AJ)] and Clinical Sciences Research and Development Merit Review Award [1I01CX001592‐01 (MH)]. Funding Information: The authors would like to thank the study participants and staff at each of the recruitment sites, including Central City Concern, CODA, Inc., De Paul Treatment Centers, Native American Rehabilitation Association of the Northwest, OutsideIn, Volunteers of America Treatment Centers, OHSU, and the Veterans Affairs Portland Health Care System Mental Health Division and Substance Abuse Treatment Program. The authors are also grateful to Alissa Bazinet, Ph.D., Matthew Arbuckle, B.S., and Bethany Winters, B.S. for their many contributions to this project as Research Assistants and Psychometrists at the MARC. The authors would also like to acknowledge the OHSU Gene Profiling Shared Resource for providing support for the qPCR assays, under the direction of Christina A. Harrington, PhD. Consultation for the analysis of the qPCR data was provided by Jack Wiedrick, M.S. at the OHSU Biostatistics & Design Program. Transmission electron microscopy was performed at the Multiscale Microscopy Core with technical support from the OHSU-FEI Living Lab and the OHSU Center for Spatial Systems Biomedicine, under the guidance of Claudia S. Lopez, PhD. The work was conducted using facilities at the VA Portland Health Care System and OHSU, Portland, Oregon. Authors JML (Research Scientist), AJ (Senior Research Career Scientist), and MH (Staff Psychologist and Neuropsychologist) acknowledge their appointments at the VA Portland Health Care System, Portland, Oregon. The contents do not represent the views of the Department of Veterans Affairs or the United States Government. This work was supported by the MARC under grant P50 DA018165 and Department of Veterans Affairs, Veterans Health Administration, Office of Research and Development, Biomedical Laboratory Research and Development Merit Review Awards [1I01BX002061 (JML), I01BX004934 (AJ)] and Clinical Sciences Research and Development Merit Review Award [1I01CX001592-01 (MH)]. Publisher Copyright: {\textcopyright} 2020 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles",
year = "2020",
month = oct,
day = "1",
doi = "10.1002/jev2.12028",
language = "English (US)",
volume = "10",
journal = "Journal of Extracellular Vesicles",
issn = "2001-3078",
publisher = "Taylor and Francis Ltd.",
number = "1",
}